Магнитные шарики Lnjnbio для набора для тотальной экстракции ДНК из кала 221005

(Метод магнитных шариков)

【Представление продукта】

This product is suitable for the rapid and efficient extraction of total DNA from stool samples. The extraction process uses Digestive Buffer and Proteinase K to lyses the intestinal flora cells in the stool, releasing the total DNA. The magnetic beads selectively adsorb the total DNA in the lysate under the action of the binding buffer. The small amount of impurities adsorbed by the magnetic beads is removed by the Washing Buffer. The magnetic beads release the adsorbed total DNA under the action of the Elution Buffer, and the high-quality total DNA of intestinal flora can be obtained. The total DNA of intestinal flora extracted by this product does not need toxic reagents such as phenol, chloroform, и т. д., and the total DNA obtained can be directly used in downstream experiments.

【Преимущества продукта】

1. High extraction yield: 10-60 μg of total DNA can be extracted from 200 mg of sample.

2. Wide applicability: suitable for solid or liquid stool samples from different sources.

3. Easy to operate: the extraction process can be completed within 30 мин, also suitable for high-throughput operation through the automated workstation.

4. High safety: free of toxic reagents such as phenol and chloroform.

【Изображение продукта】

【Экспериментальные случаи】

Случай 1:

Total DNA was extracted from six stool samples of different sources (sampling volume of 200 mg) using theStool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co. ООО. on an automated nucleic acid extraction and purification instrument, LJbio32H, and about 10 μg of total DNA could be captured with high stability.

Номер образца	А260/А280	А260/А230	Conc. (ng/μл)
1	1.76	1.91	105.6
2	1.75	1.69	82.6
3	1.77	2.39	114.9
4	1.75	1.88	107.6
56	1.77	2.43	116.4
6	1.77	2.46	115.8

Случай 2：

The same stool sample was extracted multiple times (10 repetitions) using the Stool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co., ООО. and quantitatively amplified by qPCR to detect 16S rRNA targets.

Анализ результатов：

The results showed that the Ct values for multiple extracted samples were essentially the same, thus indicating good reproducibility of the extraction kit.

【Аобласть применения】

Gut microbiome research: extract bacterial, fungal, and viral DNA / RNA, support 16S rRNA and metagenomic sequencing, and analyze the association between bacterial flora and disease.

Diagnosis of infectious diseases: detection of rotavirus, norovirus, Clostridium difficile, and other pathogens, to improve the efficiency of diarrhea pathogen identification.

Early tumor screening and precision medicine: extraction of fecal ctDNA, screening of genetic mutations and methylation markers of colorectal cancer, to achieve non-invasive early diagnosis.

Genetic diseases and metabolism research: analysis of host DNA and microflora genes, research of metabolic diseases mechanism and personalized intervention strategies.

Animal health and veterinary diagnosis: detect livestock and pet pathogens, optimize disease prevention and control, and diagnosis and treatment plan.

Environmental and food safety monitoring: tracking the pathogenic bacteria and drug resistance genes in water sources and food, to ensure public health safety.

Введение поставщика

Шанхайская биотехнологическая компания Линцзюнь., ОООбыл создан в 2016 и является профессиональным производителем биомагнитных материалов и реагентов для экстракции нуклеиновых кислот..

Мы имеем богатый опыт в экстракции и очистке нуклеиновых кислот., очистка белка, разделение клеток, хемилюминесценция и другие области техники.

Наша продукция широко используется во многих областях., например, медицинское тестирование, генетическое тестирование, университетские исследования, генетическое разведение, и так далее. Мы не только поставляем продукцию, но также можем взять на себя OEM, ОДМ, и другие потребности. Если у вас есть схожие потребности, пожалуйста, не стесняйтесь обращаться к нам по адресу sales01@lingjunbio.com.