(Método de cuentas magnéticas)

【Introducción del producto】

This product is suitable for animal muscle, hígado, bazo, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, polysaccharide, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, nucleic acid detection analysis, hibridación molecular, gene mutation detection and sequencing experiments.

【Ventajas del producto】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、High purity of extracted products, alto rendimiento, bandas electroforéticas completas

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、Seguro de usar, sin necesidad de molienda con nitrógeno líquido, el producto no contiene fenol, cloroformo, and other harmful reagents

5、Rentable, the extraction effect is comparable to that of similar products, but at a cheaper price

【Imagen del producto】

【Caso experimental】

Caso: Genomic DNA was extracted from different tissue samples of different animals with the following results:

Número de muestra	Muestra	A260/A280	A260/A230	Conc.(de/ul)	Yield（μg）	dooefficient of variation
1	20mg de camarones	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Promedio		1.8915	2.0475	113.25	11.325	0.13%
3	20mg de carne de pollo	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Promedio		1.8915	2.062	140.275	14.0275	0.55%
5	30mg de cerdo	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
Promedio		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
Promedio		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg de carne de res	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
Promedio		1.8485	1.939	90.3	9.03	0.44%
11	30mg de pescado	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Promedio		1.874	2.09	235.1	23.51	0.81%
13	3mg de corazón de pollo	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
Promedio		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
Promedio		1.8625	1.9885	134.55	13.455	3.01%
17	3mg de hígado de pescado	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
Promedio		1.7685	2.2875	370.65	37.065	0.22%
19	3mg corazón de pescado	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
Promedio		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

Camarón:

carne de pollo:

Cerdo:

Goat meat:

Carne de res:

Pez:

corazon de pollo

Hígado de pollo:

hígado de pescado:

corazón de pescado:

El electroferograma se muestra a continuación.:

Análisis de resultados:

Los resultados del ensayo de ácido ultramicronucleico (UMNA) la prueba demostró que:

①A260/A280, A260/A230, el coeficiente de variación estaba por debajo 3.62%, lo que demostró la alta estabilidad de la extracción de reactivos..

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Los rendimientos de extracción de ácidos nucleicos oscilaron entre 8-37 µg o más para diferentes muestras de tejido animal.

Los resultados de la electroforesis en gel de agarosa mostraron que:

①Various tissue samples from different animals with bright bands and good integrity.

En conclusión: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, alto rendimiento, bandas electroforéticas completas, y una cooperación uniforme y estable entre los reactivos y el instrumento.

【Aárea de aplicación】

Scientific research use

1. Genomics research: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, y otros experimentos, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. Diagnóstico de enfermedades: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, rápido, and stable nucleic acid extraction.

Introducción del proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 y es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes necesidades relacionadas, no dude en contactarnos en sales01@lingjunbio.com.