(Metode Manik Magnetik)

【Pengenalan Produk】

This product is suitable for animal muscle, liver, limpa, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, polysaccharide, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, nucleic acid detection analysis, hibridisasi molekuler, gene mutation detection and sequencing experiments.

【Keunggulan produk】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、Kemurnian tinggi dari produk yang diekstraksi, hasil tinggi, complete electrophoretic bands

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、Aman digunakan, tidak perlu penggilingan nitrogen cair, produk tidak mengandung fenol, khloroform, dan reagen berbahaya lainnya

5、Cost-effective, the extraction effect is comparable to that of similar products, but at a cheaper price

【Gambar produk】

【Kasus eksperimental】

Kasus: Genomic DNA was extracted from different tissue samples of different animals with the following results:

Nomor sampel	Mencicipi	A260/A280	A260/A230	Kesimpulan(dari/ul)	Ybidang（μg）	Coefficient of variation
1	20mg Udang	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Rata-rata		1.8915	2.0475	113.25	11.325	0.13%
3	20mg Chicken meat	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Rata-rata		1.8915	2.062	140.275	14.0275	0.55%
5	30mg Pork	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
Rata-rata		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
Rata-rata		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg Beef	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
Rata-rata		1.8485	1.939	90.3	9.03	0.44%
11	30mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Rata-rata		1.874	2.09	235.1	23.51	0.81%
13	3mg Hati ayam	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
Rata-rata		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
Rata-rata		1.8625	1.9885	134.55	13.455	3.01%
17	3mg hati ikan	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
Rata-rata		1.7685	2.2875	370.65	37.065	0.22%
19	3mg Hati ikan	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
Rata-rata		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

Shrimp:

Chicken meat:

Babi:

Goat meat:

Beef:

Fish:

Chicken heart

Chicken liver:

Fish liver:

Fish heart:

Elektroforogram ditunjukkan di bawah ini:

Analisis hasil:

Hasil Uji Asam Nukleat Ultra Mikro (UMNA) tes menunjukkan hal itu:

①A260/A280, A260/A230, the coefficient of variation was below 3.62%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 8-37 µg or more for different animal tissue samples.

Hasil elektroforesis gel agarosa menunjukkan bahwa:

①Various tissue samples from different animals with bright bands and good integrity.

Kesimpulannya: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, hasil tinggi, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【Aarea aplikasi】

Scientific research use

1. Genomics research: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, dan eksperimen lainnya, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. Diagnosis penyakit: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, fast, and stable nucleic acid extraction.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.