(Magnetische kralenmethode)

【Productintroductie】

This product is suitable for animal muscle, lever, milt, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, polysacharide, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, nucleïnezuurdetectieanalyse, moleculaire hybridisatie, gene mutation detection and sequencing experiments.

【Productvoordelen】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、Hoge zuiverheid van geëxtraheerde producten, hoge opbrengst, complete electrophoretic bands

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、Veilig in gebruik, no need for liquid nitrogen grinding, het product bevat geen fenol, chloroform, en andere schadelijke reagentia

5、Cost-effective, the extraction effect is comparable to that of similar products, but at a cheaper price

【Productafbeelding】

【Experimenteel geval】

Geval: Genomic DNA was extracted from different tissue samples of different animals with the following results:

Monsternummer	Steekproef	A260/A280	A260/A230	Conc.(van/ul)	Yield（μg）	Coefficient of variation
1	20mg garnalen	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Average		1.8915	2.0475	113.25	11.325	0.13%
3	20mg Chicken meat	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Average		1.8915	2.062	140.275	14.0275	0.55%
5	30mg varkensvlees	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
Average		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
Average		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg rundvlees	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
Average		1.8485	1.939	90.3	9.03	0.44%
11	30mg Vis	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Average		1.874	2.09	235.1	23.51	0.81%
13	3mg Chicken heart	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
Average		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
Average		1.8625	1.9885	134.55	13.455	3.01%
17	3mg Vislever	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
Average		1.7685	2.2875	370.65	37.065	0.22%
19	3mg Vishart	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
Average		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

Garnaal:

Chicken meat:

Varkensvlees:

Goat meat:

Rundvlees:

Vis:

Chicken heart

Kippenlever:

Vis lever:

Vis hart:

Het elektroferogram wordt hieronder weergegeven:

Resultaatanalyse:

De resultaten van de Ultra-Micro Nucleïnezuurtest (UMNA) proef heeft dat uitgewezen:

①A260/A280, A260/A230, the coefficient of variation was below 3.62%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 8-37 µg or more for different animal tissue samples.

De resultaten van agarosegelelektroforese lieten dat zien:

①Various tissue samples from different animals with bright bands and good integrity.

Tot slot: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, hoge opbrengst, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【Atoepassingsgebied】

Scientific research use

1. Genomics onderzoek: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, en andere experimenten, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. Ziektediagnose: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, fast, and stable nucleic acid extraction.

Introductie van leveranciers

Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 en is een professionele fabrikant van biomagnetische materialen en reagentia voor nucleïnezuurextractie.

We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie, en andere technische gebieden.

Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM uitvoeren, ODM, en andere behoeften. Als u gerelateerde behoeften heeft, Neem gerust contact met ons op via sales01@lingjunbio.com.