(Metodo della perla magnetica)

【Introduzione al prodotto】

This product is suitable for animal muscle, fegato, milza, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, polisaccaride, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, analisi di rilevamento dell'acido nucleico, ibridazione molecolare, gene mutation detection and sequencing experiments.

【Vantaggi del prodotto】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、Elevata purezza dei prodotti estratti, rendimento elevato, bande elettroforetiche complete

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、Sicuro da usare, non è necessaria la macinazione con azoto liquido, il prodotto non contiene fenoli, cloroformio, e altri reagenti dannosi

5、Conveniente, the extraction effect is comparable to that of similar products, but at a cheaper price

【Immagine del prodotto】

【Caso sperimentale】

Caso: Genomic DNA was extracted from different tissue samples of different animals with the following results:

Numero del campione	Campione	A260/A280	A260/A230	Concentrato(di/ul)	Yield（μg）	Coefficient of variation
1	20mg Gamberetti	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Media		1.8915	2.0475	113.25	11.325	0.13%
3	20mg Carne di pollo	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Media		1.8915	2.062	140.275	14.0275	0.55%
5	30mg. Maiale	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
Media		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
Media		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg di manzo	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
Media		1.8485	1.939	90.3	9.03	0.44%
11	30mg Pesce	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Media		1.874	2.09	235.1	23.51	0.81%
13	3mg Cuore di pollo	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
Media		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
Media		1.8625	1.9885	134.55	13.455	3.01%
17	3mg Fegato di pesce	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
Media		1.7685	2.2875	370.65	37.065	0.22%
19	3mg Cuore di pesce	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
Media		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

Gamberetto:

Chicken meat:

Maiale:

Goat meat:

Manzo:

Pescare:

Chicken heart

Chicken liver:

Fegato di pesce:

Cuore di pesce:

L'elettroferogramma è mostrato di seguito:

Analisi dei risultati:

I risultati del test dell'acido nucleico ultramicro (UMNA) il test lo ha dimostrato:

①A260/A280, A260/A230, il coefficiente di variazione era inferiore 3.62%, che ha dimostrato l'elevata stabilità dell'estrazione dei reagenti.

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 8-37 µg or more for different animal tissue samples.

I risultati dell’elettroforesi su gel di agarosio lo hanno dimostrato:

①Various tissue samples from different animals with bright bands and good integrity.

Insomma: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, rendimento elevato, bande elettroforetiche complete, e cooperazione uniforme e stabile tra i reagenti e lo strumento.

【UNarea di applicazione】

Scientific research use

1. Ricerca genomica: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, e altri esperimenti, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. Diagnosi della malattia: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, veloce, and stable nucleic acid extraction.

Introduzione del fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 ed è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza, e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere OEM, ODM, e altre esigenze. Se hai esigenze correlate, non esitate a contattarci all'indirizzo sales01@lingjunbio.com.