(磁気ビーズ法)

【商品紹介】

This product is suitable for animal muscle, 肝臓, 脾臓, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, 多糖類, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, nucleic acid detection analysis, 分子ハイブリダイゼーション, gene mutation detection and sequencing experiments.

【製品の利点】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、高純度の抽出物, 高収量, complete electrophoretic bands

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、安全に使用できます, no need for liquid nitrogen grinding, 製品にはフェノールが含まれていません, クロロホルム, and other harmful reagents

5、Cost-effective, the extraction effect is comparable to that of similar products, but at a cheaper price

【商品画像】

【実験事例】

場合: Genomic DNA was extracted from different tissue samples of different animals with the following results:

サンプル番号	サンプル	A260/A280	A260/A230	濃度(の/ul)	Y収量（μg）	Coefficient of variation
1	20mg エビ	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
平均		1.8915	2.0475	113.25	11.325	0.13%
3	20mg Chicken meat	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
平均		1.8915	2.062	140.275	14.0275	0.55%
5	30mg 豚肉	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
平均		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
平均		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg 牛肉	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
平均		1.8485	1.939	90.3	9.03	0.44%
11	30mg 魚	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
平均		1.874	2.09	235.1	23.51	0.81%
13	3mg 鶏ハツ	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
平均		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
平均		1.8625	1.9885	134.55	13.455	3.01%
17	3mg 魚の肝臓	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
平均		1.7685	2.2875	370.65	37.065	0.22%
19	3mg 魚の心臓	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
平均		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

エビ:

Chicken meat:

豚肉:

Goat meat:

牛肉:

魚:

Chicken heart

Chicken liver:

魚のレバー:

魚の心臓:

エレクトロフェログラムを以下に示します:

結果分析:

超微量核酸測定の結果 (ウムナ) テストでそれが示された:

①A260/A280, A260/A230, the coefficient of variation was below 3.62%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 8-37 µg or more for different animal tissue samples.

アガロースゲル電気泳動の結果、:

①Various tissue samples from different animals with bright bands and good integrity.

結論は: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, 高収量, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【あ適用範囲】

Scientific research use

1. ゲノミクス研究: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, そして他の実験, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. 病気の診断: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, 速い, and stable nucleic acid extraction.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.