Lnjnbio Magnetic Beads for Fungal DNA/RNA Extraction Kit (Magnetische kralenmethode)160889

【Productintroductie】

Samples such as mycelium, substrate and spores of fungi release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by washing solution to remove proteins, polysachariden, inorganic salt ions, enz., and finally, after elution, purified nucleic acid is obtained, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, moleculaire hybridisatie, gene mutation detection and sequencing and other experiments.

【Productvoordelen】

1, De kerngrondstoffen worden door onszelf geproduceerd, productkwaliteit en opbrengst zijn gegarandeerd.

2, The purity of the extracted product, high yield and complete electrophoretic bands make it suitable for various downstream applications.

3, Magnetic bead method of extraction, can be automated, about 20 minutes to complete the operation.

4, Safe to use, the product does not contain harmful reagents such as phenol and chloroform. Unique buffer system ensures reproducibility.

5, Versatile and suitable for extracting nucleic acids from a wide range of fungal samples such as mycelium, ascospores and spores.

【Productafbeelding】

【Experimental case】

Geval: Different fungi were sampled and DNA/RNA was extracted with the following results:

Nummer	Steekproef	A260/A280	A260/A230	Conc.(van/ul)	Yield（μg）
1	20mg Mushroom	1.901	1.905	147.75	14.775
2		1.908	1.934	149.8	14.98
Average		1.9045	1.9195	148.775	14.8775
Standarddeviation		0.0035	0.0145	1.025
CV		0.18%	0.76%	0.69%
3	10mg Needle mushroom	1.864	1.431	73.2	7.32
4		1.869	1.386	72.95	7.295
Average		1.8665	1.4085	73.075	7.3075
Standarddeviation		0.0025	0.0225	0.125
CV		0.13%	1.60%	0.17%
5	10mg Yeast dry powder	1.809	1.553	319	31.9
6		1.806	1.548	320.5	32.05
Average		1.8075	1.5505	319.75	31.975
Standarddeviation		0.0015	0.0025	0.75
CV		0.08%	0.16%	0.23%

Piekpatroondiagram:

Mushroom:

Needle mushroom：

Yeast dry powder:

Het elektroferogram wordt hieronder weergegeven:

Resultaatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①A260/A280, A260/A230, the coefficient of variation was below 2%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8-1.9, and the values of A260/A230 were above 1.4, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields were above 7 µg for different fungal samples.

The results of agarose gel electrophoresis showed that:

①Different fungal samples with bright bands and good integrity.

Tot slot: the extraction of different fungal samples using fully automated nucleic acid extraction and purification instrument showed high purity and yield of the extracted products, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【Atoepassingsgebied】

The extracted and purified nucleic acids can be used for PCR, qPCR, enzyme digestion, library construction and Southern blot.

Introductie van leveranciers

Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 en is een professionele fabrikant van biomagnetische materialen en reagentia voor nucleïnezuurextractie.

We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie en andere technische gebieden.

Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM uitvoeren, ODM, en andere behoeften. Als u gerelateerde behoeften heeft, Neem gerust contact met ons op via sales01@lingjunbio.com.