Manik Magnetik Lnjnbio untuk Kit Ekstraksi DNA/RNA Jamur (Metode Manik Magnetik)160889

【Pengenalan Produk】

Samples such as mycelium, substrate and spores of fungi release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, lalu dibilas dengan larutan pencuci untuk menghilangkan proteinnya, polisakarida, ion garam anorganik, dll., dan akhirnya, setelah elusi, purified nucleic acid is obtained, and the produk asam nukleat obtained can be used for PCR, deteksi dan analisis asam nukleat, hibridisasi molekuler, gene mutation detection and sequencing and other experiments.

【Keunggulan produk】

1, The core raw materials are produced by ourselves, product quality and yield are guaranteed.

2, The purity of the extracted product, high yield and complete electrophoretic bands make it suitable for various downstream applications.

3, Magnetic bead method of extraction, can be automated, tentang 20 minutes to complete the operation.

4, Aman digunakan, the product does not contain harmful reagents such as phenol and chloroform. Unique buffer system ensures reproducibility.

5, Versatile and suitable for extracting nucleic acids from a wide range of fungal samples such as mycelium, ascospores and spores.

【Gambar produk】

【Kasus eksperimental】

Kasus: Different fungi were sampled and DNA/RNA was extracted with the following results:

Nomor	Mencicipi	A260/A280	A260/A230	Kesimpulan(dari/ul)	Hasil（μg）
1	20mg Mushroom	1.901	1.905	147.75	14.775
2		1.908	1.934	149.8	14.98
Rata-rata		1.9045	1.9195	148.775	14.8775
Standarddeviation		0.0035	0.0145	1.025
CV		0.18%	0.76%	0.69%
3	10mg Needle mushroom	1.864	1.431	73.2	7.32
4		1.869	1.386	72.95	7.295
Rata-rata		1.8665	1.4085	73.075	7.3075
Standarddeviation		0.0025	0.0225	0.125
CV		0.13%	1.60%	0.17%
5	10mg Yeast dry powder	1.809	1.553	319	31.9
6		1.806	1.548	320.5	32.05
Rata-rata		1.8075	1.5505	319.75	31.975
Standarddeviation		0.0015	0.0025	0.75
CV		0.08%	0.16%	0.23%

Diagram pola puncak:

Jamur:

Needle mushroom：

Yeast dry powder:

Elektroforogram ditunjukkan di bawah ini:

Analisis hasil:

Hasil Uji Asam Nukleat Ultra Mikro (UMNA) tes menunjukkan hal itu:

①A260/A280, A260/A230, the coefficient of variation was below 2%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8-1.9, dan nilai A260/A230 berada di atas 1.4, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields were above 7 µg for different fungal samples.

Hasil elektroforesis gel agarosa menunjukkan bahwa:

①Different fungal samples with bright bands and good integrity.

Kesimpulannya: the extraction of different fungal samples using fully automated nucleic acid extraction and purification instrument showed high purity and yield of the extracted products, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【Aarea aplikasi】

The extracted and purified nucleic acids can be used for PCR, qPCR, pencernaan enzim, library construction and Southern blot.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.