DNA/RNA-extractiekit voor plantenzaden

 (Magnetische kralenmethode)

【Productintroductie】

This kit is specifically designed for the extraction of DNA and RNA from plant seeds and is suitable for a variety of plant seeds, including rice, tarwe, corn, enz. The lysis buffer directly lyses plant samples to release nucleic acids. Magnetic beads selectively adsorb nucleic acids from the lysate under the action of the binding buffer. Impurities are removed by washing buffer, and the nucleic acids are eluted from the beads by the elution buffer, resulting in high-quality DNA/RNA. The entire process is convenient for operation, especially suitable for automated nucleic acid extraction platforms. The extracted DNA/RNA has high yield, hoge zuiverheid, and stable quality, making it suitable for various downstream molecular biology experiments.

【Productvoordelen】

1.Effective extraction for both fresh and dried seed samples.

2.High purity and yield of extracted products with complete electrophoretic bands.

3.Broad compatibility, suitable for extracting DNA/RNA from seeds of different plant species.

4.Veilig in gebruik, geen noodzaak voor malen met vloeibare stikstof, and no harmful reagents such as phenol or chloroform.

5.Suitable for manual extraction and compatible with various high-throughput extraction instruments.

6.Fast and simple, the entire extraction process can be completed within 20-60 minutes to obtain high-purity genomic DNA/RNA.

【Productafbeelding】

【Experimentele gevallen】

Geval 1: Extraction of DNA/RNA from Different Plant Seed Samples

Nee.	Steekproef	A260/A280	A260/A230	Conc.(van/ul)	Opbrengst (µg)
1	10 mg Perilla seed	2.002	1.623	40.35	4.035
2		2.014	1.624	40.3	4.03
Gem.		2.008	1.624	40.325	4.033
3	10 mg Rapeseed	1.916	1.728	152.35	15.235
4		1.921	1.759	150.15	15.015
Gem.		1.919	1.744	151.25	15.125
5	5 mg Chinese cabbage seed	1.905	1.872	137.15	13.715
6		1.905	1.883	131.65	13.165
Gem.		1.905	1.878	134.4	13.44
7	10 mg Wheat seed	1.838	1.648	142.45	14.245
8		1.838	1.647	143.05	14.305
Gem.		1.838	1.648	142.75	14.275
9	10 mg Corn embryo	1.885	1.974	575.55	57.555
10		1.889	1.956	568.75	56.875
Gem.		1.887	1.965	572.15	57.215

Single Peak Figures:

Perilla seed:

Rapeseed:

Chinese cabbage seed:

Wheat seed:

Corn embryo:

Agarosegelelektroforese:

M: Marker (2.000 bp，1,000 bp，750 bp，500 bp，250 bp，100 bp）;

1-2： 10 mg Perilla seed 3-4： 10 mg Rapeseed

5-6： 5 mg Chinese cabbage seed 7-8： 10 mg Wheat seed

9-10： 10 mg Corn embryo

Resultaatanalyse:

NanoDrop Results:

The A260/A280 values are above 1.8, and A260/A230 values are above 1.6, indicating high purity of the extracted nucleic acids.

The yield of nucleic acids from different plant seed samples is above 4 µg.

Agarosegelelektroforese:

All samples show bright and complete bands.

【Atoepassingsgebied】

The purified nucleic acids extracted by this kit can be applied in the following areas:

1.Genomics and Transcriptomics Research:

Genomic DNA and transcriptomic RNA extraction for plant seed genome sequencing, genetic diversity analysis, klonen van genen, and gene editing validation.

High-quality total RNA suitable for transcriptome sequencing, analyse van genexpressie (bijv., RT-PCR, Real-Time RT-PCR), and Northern Blot experiments.

2.Plant Science Research:

Stress biology research: Analyzing gene expression changes in plants under stress conditions such as drought, salinity, and low temperature.

3.Agricultural Science:

Crop breeding: Extracting seed DNA for molecular marker-assisted breeding to select plant varieties with desirable traits.

GMO detection: Detecting exogenous genes in transgenic plant seeds to ensure genetic stability.

4.Clinical and Medical Research:

Pathogen detection: Extracting nucleic acids from plant samples to identify and detect viruses, bacteriën, of schimmels.

Genetic disease diagnosis: High-quality DNA extraction for genetic testing and mutation analysis.

5.High-Throughput Automation:

Compatible with automated nucleic acid extraction instruments for high-throughput sample processing, suitable for laboratories, breeding centers, and agricultural testing sites.

6.Teaching and Research:

Experimental teaching: Standard samples for molecular biology courses to develop students’ praktische vaardigheden.

Research experiments: Supporting downstream experiments such as PCR, RT-PCR, sequentiebepaling, and gene cloning.