植物種子 DNA/RNA 抽出キット

 (磁気ビーズ法)

【商品紹介】

This kit is specifically designed for the extraction of DNA and RNA from plant seeds and is suitable for a variety of plant seeds, including rice, 小麦, corn, 等. The lysis buffer directly lyses plant samples to release nucleic acids. Magnetic beads selectively adsorb nucleic acids from the lysate under the action of the binding buffer. Impurities are removed by washing buffer, and the nucleic acids are eluted from the beads by the elution buffer, resulting in high-quality DNA/RNA. The entire process is convenient for operation, especially suitable for automated nucleic acid extraction platforms. The extracted DNA/RNA has high yield, 高純度, and stable quality, making it suitable for various downstream molecular biology experiments.

【製品の利点】

1.Effective extraction for both fresh and dried seed samples.

2.High purity and yield of extracted products with complete electrophoretic bands.

3.Broad compatibility, suitable for extracting DNA/RNA from seeds of different plant species.

4.安全に使用できます, 液体窒素による粉砕は不要です, and no harmful reagents such as phenol or chloroform.

5.Suitable for manual extraction and compatible with various high-throughput extraction instruments.

6.Fast and simple, the entire extraction process can be completed within 20-60 minutes to obtain high-purity genomic DNA/RNA.

【商品画像】

【実験事例】

場合 1: Extraction of DNA/RNA from Different Plant Seed Samples

いいえ.	サンプル	A260/A280	A260/A230	濃度(の/ul)	収率 (μg)
1	10 mg Perilla seed	2.002	1.623	40.35	4.035
2		2.014	1.624	40.3	4.03
平均.		2.008	1.624	40.325	4.033
3	10 mg Rapeseed	1.916	1.728	152.35	15.235
4		1.921	1.759	150.15	15.015
平均.		1.919	1.744	151.25	15.125
5	5 mg Chinese cabbage seed	1.905	1.872	137.15	13.715
6		1.905	1.883	131.65	13.165
平均.		1.905	1.878	134.4	13.44
7	10 mg Wheat seed	1.838	1.648	142.45	14.245
8		1.838	1.647	143.05	14.305
平均.		1.838	1.648	142.75	14.275
9	10 mg Corn embryo	1.885	1.974	575.55	57.555
10		1.889	1.956	568.75	56.875
平均.		1.887	1.965	572.15	57.215

Single Peak Figures:

Perilla seed:

Rapeseed:

Chinese cabbage seed:

Wheat seed:

Corn embryo:

アガロースゲル電気泳動:

M: マーカー (2,000 bp，1,000 血圧，750 血圧，500 血圧，250 血圧，100 血圧）;

1-2： 10 mg Perilla seed 3-4： 10 mg Rapeseed

5-6： 5 mg Chinese cabbage seed 7-8： 10 mg Wheat seed

9-10： 10 mg Corn embryo

結果分析:

NanoDrop Results:

The A260/A280 values are above 1.8, and A260/A230 values are above 1.6, indicating high purity of the extracted nucleic acids.

The yield of nucleic acids from different plant seed samples is above 4 μg.

アガロースゲル電気泳動:

All samples show bright and complete bands.

【あ適用範囲】

The purified nucleic acids extracted by this kit can be applied in the following areas:

1.ゲノミクスとトランスクリプトミクスの研究:

Genomic DNA and transcriptomic RNA extraction for plant seed genome sequencing, genetic diversity analysis, 遺伝子クローニング, and gene editing validation.

High-quality total RNA suitable for transcriptome sequencing, 遺伝子発現解析 (例えば, RT-PCR, Real-Time RT-PCR), and Northern Blot experiments.

2.植物科学研究:

Stress biology research: Analyzing gene expression changes in plants under stress conditions such as drought, 塩分, and low temperature.

3.Agricultural Science:

Crop breeding: Extracting seed DNA for molecular marker-assisted breeding to select plant varieties with desirable traits.

GMO detection: Detecting exogenous genes in transgenic plant seeds to ensure genetic stability.

4.Clinical and Medical Research:

Pathogen detection: Extracting nucleic acids from plant samples to identify and detect viruses, 細菌, または菌類.

Genetic disease diagnosis: High-quality DNA extraction for genetic testing and mutation analysis.

5.High-Throughput Automation:

Compatible with automated nucleic acid extraction instruments for high-throughput sample processing, suitable for laboratories, breeding centers, and agricultural testing sites.

6.Teaching and Research:

Experimental teaching: Standard samples for molecular biology courses to develop students’ 実践的なスキル.

Research experiments: Supporting downstream experiments such as PCR, RT-PCR, 順序付け, and gene cloning.