مجموعة أدوات استخراج الحمض النووي/الحمض النووي الريبي (DNA) من بذور النباتات

 (طريقة الخرزة المغناطيسية)

【مقدمة المنتج】

This kit is specifically designed for the extraction of DNA and RNA from plant seeds and is suitable for a variety of plant seeds, including rice, wheat, corn, إلخ. The lysis buffer directly lyses plant samples to release nucleic acids. Magnetic beads selectively adsorb nucleic acids from the lysate under the action of the binding buffer. Impurities are removed by washing buffer, and the nucleic acids are eluted from the beads by the elution buffer, resulting in high-quality DNA/RNA. The entire process is convenient for operation, especially suitable for automated nucleic acid extraction platforms. The extracted DNA/RNA has high yield, درجة نقاء عالية, and stable quality, making it suitable for various downstream molecular biology experiments.

【مزايا المنتج】

1.Effective extraction for both fresh and dried seed samples.

2.High purity and yield of extracted products with complete electrophoretic bands.

3.Broad compatibility, suitable for extracting DNA/RNA from seeds of different plant species.

4.آمنة للاستخدام, no need for liquid nitrogen grinding, and no harmful reagents such as phenol or chloroform.

5.Suitable for manual extraction and compatible with various high-throughput extraction instruments.

6.Fast and simple, the entire extraction process can be completed within 20-60 minutes to obtain high-purity genomic DNA/RNA.

【صورة المنتج】

【حالات تجريبية】

قضية 1: Extraction of DNA/RNA from Different Plant Seed Samples

لا.	عينة	A260/A280	A260/A230	Conc.(من / ماي)	أَثْمَر (ميكروغرام)
1	10 mg Perilla seed	2.002	1.623	40.35	4.035
2		2.014	1.624	40.3	4.03
متوسط.		2.008	1.624	40.325	4.033
3	10 mg Rapeseed	1.916	1.728	152.35	15.235
4		1.921	1.759	150.15	15.015
متوسط.		1.919	1.744	151.25	15.125
5	5 mg Chinese cabbage seed	1.905	1.872	137.15	13.715
6		1.905	1.883	131.65	13.165
متوسط.		1.905	1.878	134.4	13.44
7	10 mg Wheat seed	1.838	1.648	142.45	14.245
8		1.838	1.647	143.05	14.305
متوسط.		1.838	1.648	142.75	14.275
9	10 mg Corn embryo	1.885	1.974	575.55	57.555
10		1.889	1.956	568.75	56.875
متوسط.		1.887	1.965	572.15	57.215

Single Peak Figures:

Perilla seed:

Rapeseed:

Chinese cabbage seed:

Wheat seed:

Corn embryo:

الاغاروز هلام الكهربائي:

م: علامة (2000 سنة مضت，1,000 بي بي，750 بي بي，500 بي بي，250 بي بي，100 نقطة أساس);

1-2： 10 mg Perilla seed 3-4： 10 mg Rapeseed

5-6： 5 mg Chinese cabbage seed 7-8： 10 mg Wheat seed

9-10： 10 mg Corn embryo

تحليل النتيجة:

NanoDrop Results:

The A260/A280 values are above 1.8, and A260/A230 values are above 1.6, indicating high purity of the extracted nucleic acids.

The yield of nucleic acids from different plant seed samples is above 4 ميكروغرام.

الاغاروز هلام الكهربائي:

All samples show bright and complete bands.

【أمنطقة التطبيق 】

The purified nucleic acids extracted by this kit can be applied in the following areas:

1.أبحاث الجينوم والنسخ:

Genomic DNA and transcriptomic RNA extraction for plant seed genome sequencing, genetic diversity analysis, استنساخ الجينات, and gene editing validation.

High-quality total RNA suitable for transcriptome sequencing, تحليل التعبير الجيني (على سبيل المثال, RT-PCR, Real-Time RT-PCR), and Northern Blot experiments.

2.أبحاث علوم النبات:

Stress biology research: Analyzing gene expression changes in plants under stress conditions such as drought, الملوحة, and low temperature.

3.Agricultural Science:

Crop breeding: Extracting seed DNA for molecular marker-assisted breeding to select plant varieties with desirable traits.

GMO detection: Detecting exogenous genes in transgenic plant seeds to ensure genetic stability.

4.Clinical and Medical Research:

Pathogen detection: Extracting nucleic acids from plant samples to identify and detect viruses, البكتيريا, أو الفطريات.

Genetic disease diagnosis: High-quality DNA extraction for genetic testing and mutation analysis.

5.High-Throughput Automation:

Compatible with automated nucleic acid extraction instruments for high-throughput sample processing, suitable for laboratories, breeding centers, and agricultural testing sites.

6.Teaching and Research:

Experimental teaching: Standard samples for molecular biology courses to develop students’ المهارات العملية.

Research experiments: Supporting downstream experiments such as PCR, RT-PCR, التسلسل, and gene cloning.