தாவர விதை DNA/RNA பிரித்தெடுக்கும் கருவி

 (காந்த மணி முறை)

【தயாரிப்பு அறிமுகம்】

This kit is specifically designed for the extraction of DNA and RNA from plant seeds and is suitable for a variety of plant seeds, including rice, wheat, corn, முதலியன. The lysis buffer directly lyses plant samples to release nucleic acids. Magnetic beads selectively adsorb nucleic acids from the lysate under the action of the binding buffer. Impurities are removed by washing buffer, and the nucleic acids are eluted from the beads by the elution buffer, resulting in high-quality DNA/RNA. The entire process is convenient for operation, especially suitable for automated nucleic acid extraction platforms. The extracted DNA/RNA has high yield, உயர் தூய்மை, and stable quality, making it suitable for various downstream molecular biology experiments.

【தயாரிப்பு நன்மைகள்】

1.Effective extraction for both fresh and dried seed samples.

2.High purity and yield of extracted products with complete electrophoretic bands.

3.Broad compatibility, suitable for extracting DNA/RNA from seeds of different plant species.

4.Safe to use, no need for liquid nitrogen grinding, and no harmful reagents such as phenol or chloroform.

5.Suitable for manual extraction and compatible with various high-throughput extraction instruments.

6.Fast and simple, the entire extraction process can be completed within 20-60 minutes to obtain high-purity genomic DNA/RNA.

【தயாரிப்பு படம்】

【பரிசோதனை வழக்குகள்】

வழக்கு 1: Extraction of DNA/RNA from Different Plant Seed Samples

இல்லை.	மாதிரி	A260/A280	A260/A230	ஒப்பந்தம்(இன்/உல்)	மகசூல் (µg)
1	10 mg Perilla seed	2.002	1.623	40.35	4.035
2		2.014	1.624	40.3	4.03
சராசரி.		2.008	1.624	40.325	4.033
3	10 mg Rapeseed	1.916	1.728	152.35	15.235
4		1.921	1.759	150.15	15.015
சராசரி.		1.919	1.744	151.25	15.125
5	5 mg Chinese cabbage seed	1.905	1.872	137.15	13.715
6		1.905	1.883	131.65	13.165
சராசரி.		1.905	1.878	134.4	13.44
7	10 mg Wheat seed	1.838	1.648	142.45	14.245
8		1.838	1.647	143.05	14.305
சராசரி.		1.838	1.648	142.75	14.275
9	10 mg Corn embryo	1.885	1.974	575.55	57.555
10		1.889	1.956	568.75	56.875
சராசரி.		1.887	1.965	572.15	57.215

Single Peak Figures:

Perilla seed:

Rapeseed:

Chinese cabbage seed:

Wheat seed:

Corn embryo:

அகரோஸ் ஜெல் எலக்ட்ரோபோரேசிஸ்:

எம்: Marker（2,000 bp，1,000 பிபி，750 பிபி，500 பிபி，250 பிபி，100 bp);

1-2： 10 mg Perilla seed 3-4： 10 mg Rapeseed

5-6： 5 mg Chinese cabbage seed 7-8： 10 mg Wheat seed

9-10： 10 mg Corn embryo

முடிவு பகுப்பாய்வு:

NanoDrop Results:

The A260/A280 values are above 1.8, and A260/A230 values are above 1.6, indicating high purity of the extracted nucleic acids.

The yield of nucleic acids from different plant seed samples is above 4 µg.

அகரோஸ் ஜெல் எலக்ட்ரோபோரேசிஸ்:

All samples show bright and complete bands.

【ஏவிண்ணப்பப் பகுதி】

The purified nucleic acids extracted by this kit can be applied in the following areas:

1.Genomics and Transcriptomics Research:

Genomic DNA and transcriptomic RNA extraction for plant seed genome sequencing, genetic diversity analysis, gene cloning, and gene editing validation.

High-quality total RNA suitable for transcriptome sequencing, மரபணு வெளிப்பாடு பகுப்பாய்வு (எ.கா., RT-PCR, Real-Time RT-PCR), and Northern Blot experiments.

2.தாவர அறிவியல் ஆராய்ச்சி:

Stress biology research: Analyzing gene expression changes in plants under stress conditions such as drought, உப்புத்தன்மை, and low temperature.

3.Agricultural Science:

Crop breeding: Extracting seed DNA for molecular marker-assisted breeding to select plant varieties with desirable traits.

GMO detection: Detecting exogenous genes in transgenic plant seeds to ensure genetic stability.

4.Clinical and Medical Research:

Pathogen detection: Extracting nucleic acids from plant samples to identify and detect viruses, பாக்டீரியா, or fungi.

Genetic disease diagnosis: High-quality DNA extraction for genetic testing and mutation analysis.

5.High-Throughput Automation:

Compatible with automated nucleic acid extraction instruments for high-throughput sample processing, suitable for laboratories, breeding centers, and agricultural testing sites.

6.Teaching and Research:

Experimental teaching: Standard samples for molecular biology courses to develop students’ practical skills.

Research experiments: Supporting downstream experiments such as PCR, RT-PCR, வரிசைப்படுத்துதல், and gene cloning.