Manik Magnetik Lnjnbio untuk Kit Pengekstrakan DNA Tisu FFPE 221006

（Magnetic Beads Method）

【Pengenalan Produk】

The paraffin-embedded tissue DNA extraction reagent uses an environmentally friendly deparaffinization method to remove paraffin, and special lysis conditions are applied to release the DNA in the tissue section, overcoming the inhibition effect caused by formalin cross-linking. The reagent uses DNA-binding magnetic beads and a unique buffer system to purify high-quality DNA while maximizing the removal of proteins, inorganic salts and other organic impurities. The obtained DNA has good integrity, kesucian yang tinggi, kualiti yang stabil dan boleh dipercayai, and is suitable for downstream experiments such as PCR, qPCR, SNP gene analysis, dan urutan.

【Kelebihan produk】

1. Selamat dan tidak toksik: no need for xylene dewaxing, no need for toxic organic reagents such as phenol/chloroform

2. Excellent applicability: suitable for paraffin sections of various tissues

3. Kesucian yang tinggi: effectively remove PCR inhibitors such as proteins and inorganic salts, which is convenient for downstream experiments.

4. This kit can be used for manual extraction, and can also be integrated with a variety of high-throughput extractors.

【Imej produk】

 

【Kes eksperimen】

Kes1：

The DNA of 10 cases of paraffin-embedded tissues (5 μm, 6 sections) of human gastric mucosa was extracted by using our company’s paraffin-embedded tissue DNA extraction kit, and then qPCR was performed with human GAPDH housekeeper gene as the target gene, and whether the extracted product affected qPCR amplification.

Analisis keputusan: The results showed that the amplification curve was S-shaped, and there was no obvious inhibition. The results show that the DNA extracted by this reagent is of excellent quality and can be directly used in downstream PCR and other follow-up experiments.

Case2：

The DNA of paraffin-embedded tissue DNA extraction reagent was used to extract the paraffin-embedded tissue (20 μm, 1 sections) of pig lung, canine pancreas, and chicken lung, and the extracted products were tested by ultra-micro-ultraviolet-visible spectrophotometer, dan hasilnya adalah seperti berikut:

Tidak.	Sampel	A260/A280	A260/A230	Conc.(ng/μL)	Yield（μg）
1	Porcine lung embedded tissue sections	1.75	2.104	297.1	29.71
2		1.76	2.115	280.1	28.01
3	Canine pancreatic embedded tissue sections	1.754	2.089	158.4	15.84
4		1.709	2.083	195.55	19.555
5	Chicken lung embedded tissue sections	1.616	1.946	114.8	11.48
6		1.606	1.826	108.55	10.855

Keputusan: The results showed that the reagent could stably extract DNA with good purity and high yield for different types of embedded tissues, which could be directly used in downstream PCR and other follow-up experiments.

【Kawasan permohonan】

The purified DNA extracted from this kit can be directly used in downstream molecular biology experiments such as PCR, qPCR, dPCR, NGS, SNP gene analysis, STR gene identification, pathological molecular research, dll., and can be applied to identification, diagnosis penyakit, personalized medicine and other fields.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.