대용량 시료용 엔도톡신 프리 플라스미드 DNA 추출 키트 241001L

(마그네틱 비드 방식)

【제품소개】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, 독특한 솔루션 포뮬러를 사용하면 엔도톡신을 제거하기 위해 몇 번의 간단한 세척만 필요합니다., 단백질, 다당류 및 기타 불순물, 고품질의 플라스미드 DNA를 얻기 위해. The extraction and purification of plasmid DNA using this product cause minimal damage to the plasmid, resulting in high concentration and a high proportion of supercoiled DNA. The plasmid DNA extracted by this product is suitable for downstream applications that are sensitive to endotoxins, such as transfection experiments, gene therapy, and in vitro transcription.

【제품의 장점】

1.Core Materials Self-Produced: The core raw materials are manufactured in-house, ensuring both the quality and yield of the product.

2. High-Purity Endotoxin-Free: The extracted plasmid DNA has extremely low endotoxin content, making it suitable for cell transfection and in vivo experiments.
3. Efficient Extraction: The magnetic bead method efficiently binds plasmid DNA, achieving high extraction efficiency and is suitable for large-scale extraction (up to 1 mg or more).
4. Fast and Convenient: The extraction process can be completed within 1 hour and can be automated using a fully automatic nucleic acid extraction and purification instrument, enabling high-throughput operations.
5. Broad Applicability: Suitable for the extraction of both high-copy and low-copy plasmids.
6. 높은 수율과 순도: 에서 100 mL of bacterial culture (low-copy plasmid), a yield of up to 1.17 mg can be achieved. The OD260/OD280 ratio is typically between 1.7 그리고 2.0, with a high proportion of supercoiled DNA.
7. 다운스트림 호환성: The extracted plasmid DNA is ready for downstream applications such as transfection, 시퀀싱, PCR, and restriction enzyme digestion.
8. Simple, 빠른, and Safe: The operation is straightforward and rapid, without the need for toxic organic reagents such as phenol or chloroform.

【상품 이미지】

【실험사례】

사례: 

The sample is DH 5α 이자형. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 bp, low copy plasmid), bacterial solution OD₆₀₀ = 0.103. 피lasmid was extracted ~에 의해 LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). 에이fter extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

숫자	Bacterial Solution Volume(밀리리터)	에이260/A280	에이260/A230	농도(ng/μL)	이자형lution 다섯olume (중엘)	생산하다(중g)
1	100	1.756	1.99	464.95	1	1.162
2		1.757	1.996	468.15		1.170
평균		1.756	1.993	466.55		1.166
3	200	1.819	2.053	840.35		2.100
4		1.818	2.059	830.55		2.076
평균		1.819	2.056	835.45		2.089

The peak patterns are as follows:

전기영동 결과는 다음과 같습니다::

메모: the original concentration of 6 μL on the sample electrophoresis, 아가로스 겔 농도 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of A₂₆₀ / 에이₂₈₀ is 1.75-1.81 and A₂₆₀ / 에이₂₃₀ ~이다 1.9-2.0, 추출된 제품의 높은 순도를 입증.

② Under OD₆₀₀ = 0.103, the yield of 100 mL/200mL of culture solution can reach about 1.166 mg/2.1 mg, 각기.

아가로스겔 전기영동 결과는 다음과 같다.:

밴드는 밝고 완전하다..

결론적으로, 100 mL and 200 mL의 엔도프리 DNA를 플라스미드에서 추출했습니다., 고순도 제품 생산, 높은 수율과 완전한 전기영동 밴드.

【적용분야】

The plasmid DNA extracted using this kit can be applied in the following research fields:

1. Transfection and Cell Culture: The extracted plasmid DNA has low endotoxin levels, making it suitable for applications such as transfection of eukaryotic cells and in vitro transcription.

2.  Molecular Biology Experiments: Suitable for routine operations such as restriction enzyme digestion, PCR, 시퀀싱, 결찰, 그리고 변신.

3. High-Throughput Screening: Supports automated extraction, ideal for the construction and screening of large-scale plasmid libraries.

4. Clinical and Research Applications: Suitable for applications with extremely high requirements for plasmid purity, such as gene therapy and vaccine development.

공급업체 소개

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련 요구사항이 있는 경우, 언제든지 sales01@lingjunbio.com으로 문의해 주세요..