Trealamh Eastóscadh DNA Plasmid saor ó Endotoxin le haghaidh Samplaí Móra 241001L

(Modh Coirnín Maighnéadach)

【Réamhrá Táirge】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, ní gá ach cúpla glanadh simplí a úsáid chun an endotoxin a bhaint, próitéin, polaisiúicríd agus neamhíonachtaí eile, DNA plasmid ardchaighdeáin a fháil. The extraction and purification of plasmid DNA using this product cause minimal damage to the plasmid, resulting in high concentration and a high proportion of supercoiled DNA. The plasmid DNA extracted by this product is suitable for downstream applications that are sensitive to endotoxins, such as transfection experiments, gene therapy, and in vitro transcription.

【Buntáistí táirge】

1.Core Materials Self-Produced: The core raw materials are manufactured in-house, ensuring both the quality and yield of the product.

2. High-Purity Endotoxin-Free: The extracted plasmid DNA has extremely low endotoxin content, making it suitable for cell transfection and in vivo experiments.
3. Efficient Extraction: The magnetic bead method efficiently binds plasmid DNA, achieving high extraction efficiency and is suitable for large-scale extraction (suas go dtí 1 mg or more).
4. Fast and Convenient: The extraction process can be completed within 1 hour and can be automated using a fully automatic nucleic acid extraction and purification instrument, enabling high-throughput operations.
5. Broad Applicability: Suitable for the extraction of both high-copy and low-copy plasmids.
6. Toradh Ard agus Íonacht: Ó 100 mL of bacterial culture (low-copy plasmid), a yield of up to 1.17 mg can be achieved. The OD260/OD280 ratio is typically between 1.7 agus 2.0, with a high proportion of supercoiled DNA.
7. Comhoiriúnacht le sruth: The extracted plasmid DNA is ready for downstream applications such as transfection, seicheamh, PCR, and restriction enzyme digestion.
8. Simple, Go tapa, and Safe: The operation is straightforward and rapid, without the need for toxic organic reagents such as phenol or chloroform.

【Íomhá táirge】

【Cásanna turgnamhacha】

Cás: 

Is é an sampla DH 5α E. coli (ina bhfuil PHY300PLK-egls géine plasmid, bonn péire faoi 6000 bp, plasmid cóip íseal), tuaslagán baictéarach OD₆₀₀ = 0.103. pbaineadh lasmid le LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). Atar éis eastóscadh a chinneadh ag speictreafótaiméadar infheicthe ultraivialait rian Quawell Q5000, tá na torthaí mar seo a leanas:

Uimhir	Imleabhar Réiteach Baictéarach(mL)	A260/A280	A260/A230	Conc.(ng/μL)	Eluadh Volume (mL)	Toradh(mg)
1	100	1.756	1.99	464.95	1	1.162
2		1.757	1.996	468.15		1.170
Meán		1.756	1.993	466.55		1.166
3	200	1.819	2.053	840.35		2.100
4		1.818	2.059	830.55		2.076
Meán		1.819	2.056	835.45		2.089

Is iad seo a leanas na patrúin buaic:

Is iad seo a leanas na torthaí leictreafóiréis::

Nóta: an tiúchan bunaidh de 6 μL ar an leictreafóiréis samplach, tiúchan glóthach agarose de 1%, 120 V leictreafóiréis.

(2) Anailís ar thorthaí:

Quawell Q5000 Léiríonn torthaí speictreafótaiméadar infheicthe micrea-ultraivialait go bhfuil:

① The value of A₂₆₀ / A₂₈₀ is 1.75-1.81 and A₂₆₀ / A₂₃₀ tá 1.9-2.0, a léiríonn íonacht ard an táirge asbhainte.

② Faoi OD₆₀₀ = 0.103, the yield of 100 mL/200mL of culture solution can reach about 1.166 mg/2.1 mg, faoi ​​seach.

Léirigh torthaí an leictreafóiréis glóthach agarose ansin é sin:

Tá na bannaí geal agus iomlán.

Mar fhocal scoir, 100 mL and 200 Baineadh ml de DNA endofree as plasmid, ag táirgeadh na dtáirgí a bhfuil íonacht ard acu, táirgeacht ard agus bannaí electrophoretic iomlán.

【Limistéar iarratais】

The plasmid DNA extracted using this kit can be applied in the following research fields:

1. Transfection and Cell Culture: The extracted plasmid DNA has low endotoxin levels, making it suitable for applications such as transfection of eukaryotic cells and in vitro transcription.

2.  Molecular Biology Experiments: Suitable for routine operations such as restriction enzyme digestion, PCR, seicheamh, ligation, agus claochlú.

3. High-Throughput Screening: Supports automated extraction, ideal for the construction and screening of large-scale plasmid libraries.

4. Clinical and Research Applications: Suitable for applications with extremely high requirements for plasmid purity, such as gene therapy and vaccine development.

Réamhrá Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 agus is monaróir gairmiúil ábhair bhithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, chemiluminescence agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní hamháin go gcuirimid táirgí ar fáil ach is féidir linn OEM a dhéanamh freisin, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn ag sales01@lingjunbio.com.