Kit d’extraction d’ADN plasmidique sans endotoxine pour échantillons à grand volume 241001L

(Méthode des billes magnétiques)

【Présentation du produit】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only needs a few simple cleaning to remove endotoxin, protéine, polysaccharide et autres impuretés, pour obtenir un ADN plasmidique de haute qualité. The extraction and purification of plasmid DNA using this product cause minimal damage to the plasmid, resulting in high concentration and a high proportion of supercoiled DNA. The plasmid DNA extracted by this product is suitable for downstream applications that are sensitive to endotoxins, such as transfection experiments, gene therapy, and in vitro transcription.

【Avantages du produit】

1.Core Materials Self-Produced: The core raw materials are manufactured in-house, ensuring both the quality and yield of the product.

2. High-Purity Endotoxin-Free: The extracted plasmid DNA has extremely low endotoxin content, making it suitable for cell transfection and in vivo experiments.
3. Efficient Extraction: The magnetic bead method efficiently binds plasmid DNA, achieving high extraction efficiency and is suitable for large-scale extraction (jusqu'à 1 mg or more).
4. Fast and Convenient: The extraction process can be completed within 1 hour and can be automated using a fully automatic nucleic acid extraction and purification instrument, enabling high-throughput operations.
5. Broad Applicability: Suitable for the extraction of both high-copy and low-copy plasmids.
6. Rendement élevé et pureté: Depuis 100 mL of bacterial culture (low-copy plasmid), a yield of up to 1.17 mg can be achieved. The OD260/OD280 ratio is typically between 1.7 et 2.0, with a high proportion of supercoiled DNA.
7. Compatibilité en aval: The extracted plasmid DNA is ready for downstream applications such as transfection, séquençage, RAP, and restriction enzyme digestion.
8. Simple, Fast, and Safe: The operation is straightforward and rapid, without the need for toxic organic reagents such as phenol or chloroform.

【Image du produit】

【Cas expérimentaux】

Cas: 

The sample is DH 5α E. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 pb, low copy plasmid), bacterial solution OD₆₀₀ = 0.103. P.lasmid was extracted par LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). UNfter extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

Nombre	Bacterial Solution Volume(ml)	UN260/A280	UN260/A230	Conc.(ng/μL)	Elution Volume (mL)	Rendement(mg)
1	100	1.756	1.99	464.95	1	1.162
2		1.757	1.996	468.15		1.170
Moyenne		1.756	1.993	466.55		1.166
3	200	1.819	2.053	840.35		2.100
4		1.818	2.059	830.55		2.076
Moyenne		1.819	2.056	835.45		2.089

The peak patterns are as follows:

The electrophoresis results are as follows::

Note: the original concentration of 6 μL on the sample electrophoresis, concentration de gel d'agarose de 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of A₂₆₀ / UN₂₈₀ is 1.75-1.81 and A₂₆₀ / UN₂₃₀ est 1.9-2.0, démontrant la grande pureté du produit extrait.

② Under OD₆₀₀ = 0.103, the yield of 100 mL/200mL of culture solution can reach about 1.166 mg/2.1 mg, respectivement.

Les résultats de l'électrophorèse sur gel d'agarose ont ensuite montré que:

The bands are bright and complete.

En conclusion, 100 mL and 200 mL of endofree DNA was extracted from plasmid, producing the products with high purity, bandes électrophorétiques complètes et à haut rendement.

【Domaine d'application】

The plasmid DNA extracted using this kit can be applied in the following research fields:

1. Transfection and Cell Culture: The extracted plasmid DNA has low endotoxin levels, making it suitable for applications such as transfection of eukaryotic cells and in vitro transcription.

2.  Expériences de biologie moléculaire: Suitable for routine operations such as restriction enzyme digestion, RAP, séquençage, ligature, and transformation.

3. High-Throughput Screening: Supports automated extraction, ideal for the construction and screening of large-scale plasmid libraries.

4. Clinical and Research Applications: Suitable for applications with extremely high requirements for plasmid purity, such as gene therapy and vaccine development.

Présentation du fournisseur

Société de biotechnologie Shanghai Lingjun., Ltd.a été établi en 2016 et est un fabricant professionnel de matériaux biomagnétiques et de réactifs d'extraction d'acide nucléique.

Nous avons une riche expérience dans l'extraction et la purification des acides nucléiques, purification des protéines, séparation cellulaire, chimiluminescence et autres domaines techniques.

Nos produits sont largement utilisés dans de nombreux domaines, comme les tests médicaux, tests génétiques, recherche universitaire, sélection génétique, et ainsi de suite. Nous fournissons non seulement des produits, mais pouvons également entreprendre des OEM, ODM, et autres besoins. Si vous avez des besoins connexes, n'hésitez pas à nous contacter à sales01@lingjunbio.com.