Lnjnbio Perlas magnéticas para hisopos Kit de extracción de ácido nucleico 221004(Método de cuentas magnéticas)

【Introducción del producto】

This product is mainly used for the extraction, enrichment and purification steps of nucleic acid in swab wash, solución de preservación de virus, saliva, semen, orina, lymph fluid and many other body fluid samples and animal tissue cell homogenate. The sample is lysed and released nucleic acid under the action of guanidine salt, the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, then rinsed by washing solution to remove protein and residual guanidinium salt, etc., and finally eluted to get purified nucleic acid, and the obtained producto de ácido nucleico can be used in experiments such as PCR amplification, detección y análisis de ácidos nucleicos, detección de mutaciones genéticas, y secuenciación.

【Ventajas del producto】

1、 Lysis and binding with one step, after adding the sample can be on the machine for extraction, the extraction process does not need to pause to add other reagents.

2、The extracted nucleic acid has high purity and good integrity.

3、Efficient and quick, with automatic nucleic acid extractor, solo 9 minutes to complete the extraction process.

4、No es necesario añadir proteinasa K en el proceso de extracción., and it can be transported and stored at room temperature.

5、The reagent system has good accuracy, good reproducibility and high sensitivity.

6、Gran versatilidad, Se puede utilizar con diferentes fabricantes de equipos automatizados de extracción de ácidos nucleicos..

【Imagen del producto】

【Caso experimental】

A coronavirus pseudovirus was extracted on a fully automated nucleic acid extractor LJbio32H using swab-based nucleic acid extraction reagents from Shanghai Leader Biotechnology Co. The extracted products were subjected to qPCR, and the data related to the precision and sensitivity were as follows:

The results showed that the amplification curves of both high and low concentration samples showed good reproducibility. 20 samples with the lowest detection limits could be detected stably.

【Área de aplicación】

1、Detection of pathogens of respiratory tract infections

Virus screening: rapid extraction of SARS-CoV-2, virus de la influenza (A / B), Virus sincitial respiratorio (RSV), and adenovirus from nasopharyngeal swabs and oropharyngeal swabs to support PCR, thermostatic amplification and other molecular tests.

Bacterial identification: for B. pertussis, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens, efficiently enrich trace DNA, and improve the detection rate of culture-negative samples.

Mixed infection analysis: compatible with multiplex PCR or metagenomic sequencing (mNGS), synchronously extracting nucleic acids of a variety of pathogens from a single swab, to solve the pathogen diagnosis problem of difficult pneumonia and severe infection.

2. Neonatal and maternal and infant health monitoring

Group B Streptococcus (GBS) screening: GBS DNA from late perianal swabs to prevent neonatal sepsis and meningitis.

Congenital infection screening: for cytomegalovirus (CMV), herpes simplex virus (HSV), neonatal samples can be non-invasively obtained through oral swabs for early diagnosis of congenital infection.

3. Prevention and control of zoonoses and animal diseases

Avian influenza surveillance: viral RNA was extracted from avian throat / cloaca swabs to monitor H5N1 and H7N9 subtypes in real time.

Pet pathogen detection: used for the diagnosis of pet respiratory / digestive tract infections, such as canine distemper and cat calicivirus, to ensure the prevention and control of zoonosis.

Wildlife traceability: Virus genome data was obtained from wildlife oral swabs to support the source tracing study of emerging infectious diseases (mi. gramo., Ebola, MERS-CoV).

4. Environmental and food hygiene monitoring

Cold chain detection: Novel Coronavirus RNA is extracted from object surface swabs to monitor the pollution risk of imported cold chain food.

Hospital infection control: extract the resistant bacterial DNA (mi. gramo. MRSA, CRE) from the surface swab of ICU equipment to track the chain of nosocomial transmission.

Seguridad alimentaria: detect food-borne pathogenic bacteria such as Salmonella and Listeria in food processing swabs to ensure the safety of the whole industry chain.

Introducción del proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 y es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes necesidades relacionadas, no dude en contactarnos en sales01@lingjunbio.com.