Lnjnbio 綿棒用磁気ビーズ核酸抽出キット 221004(磁気ビーズ法)

【商品紹介】

This product is mainly used for the extraction, enrichment and purification steps of nucleic acid in swab wash, ウイルス保存液, 唾液, 精液, 尿, lymph fluid and many other body fluid samples and animal tissue cell homogenate. The sample is lysed and released nucleic acid under the action of guanidine salt, the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, then rinsed by washing solution to remove protein and residual guanidinium salt, 等, and finally eluted to get purified nucleic acid, and the obtained nucleic acid product can be used in experiments such as PCR amplification, 核酸の検出と分析, 遺伝子変異の検出, そしてシーケンス.

【製品の利点】

1、 Lysis and binding with one step, after adding the sample can be on the machine for extraction, the extraction process does not need to pause to add other reagents.

2、The extracted nucleic acid has high purity and good integrity.

3、Efficient and quick, with automatic nucleic acid extractor, のみ 9 minutes to complete the extraction process.

4、No need to add proteinase K in the extraction process, and it can be transported and stored at room temperature.

5、The reagent system has good accuracy, good reproducibility and high sensitivity.

6、強力な汎用性, can be used with different manufacturers of automated nucleic acid extraction equipment.

【商品画像】

【実験事例】

A coronavirus pseudovirus was extracted on a fully automated nucleic acid extractor LJbio32H using swab-based nucleic acid extraction reagents from Shanghai Leader Biotechnology Co. The extracted products were subjected to qPCR, and the data related to the precision and sensitivity were as follows:

The results showed that the amplification curves of both high and low concentration samples showed good reproducibility. 20 samples with the lowest detection limits could be detected stably.

【適用範囲】

1、Detection of pathogens of respiratory tract infections

Virus screening: rapid extraction of SARS-CoV-2, インフルエンザウイルス (あ / B), RSウイルス (RSV), and adenovirus from nasopharyngeal swabs and oropharyngeal swabs to support PCR, thermostatic amplification and other molecular tests.

Bacterial identification: for B. pertussis, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens, efficiently enrich trace DNA, and improve the detection rate of culture-negative samples.

Mixed infection analysis: compatible with multiplex PCR or metagenomic sequencing (mNGS), synchronously extracting nucleic acids of a variety of pathogens from a single swab, to solve the pathogen diagnosis problem of difficult pneumonia and severe infection.

2. Neonatal and maternal and infant health monitoring

Group B Streptococcus (GBS) screening: GBS DNA from late perianal swabs to prevent neonatal sepsis and meningitis.

Congenital infection screening: for cytomegalovirus (CMV), herpes simplex virus (HSV), neonatal samples can be non-invasively obtained through oral swabs for early diagnosis of congenital infection.

3. Prevention and control of zoonoses and animal diseases

Avian influenza surveillance: viral RNA was extracted from avian throat / cloaca swabs to monitor H5N1 and H7N9 subtypes in real time.

Pet pathogen detection: used for the diagnosis of pet respiratory / digestive tract infections, such as canine distemper and cat calicivirus, to ensure the prevention and control of zoonosis.

Wildlife traceability: Virus genome data was obtained from wildlife oral swabs to support the source tracing study of emerging infectious diseases (e. g., Ebola, MERS-CoV).

4. Environmental and food hygiene monitoring

Cold chain detection: Novel Coronavirus RNA is extracted from object surface swabs to monitor the pollution risk of imported cold chain food.

Hospital infection control: extract the resistant bacterial DNA (e. g. MRSA, CRE) from the surface swab of ICU equipment to track the chain of nosocomial transmission.

食品の安全性: detect food-borne pathogenic bacteria such as Salmonella and Listeria in food processing swabs to ensure the safety of the whole industry chain.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, sales01@lingjunbio.comまでお気軽にお問い合わせください。.