Teknologi Pengurutan Nanopore: Itu “Kunci Generasi Keempat” untuk Membuka Misteri Gen

Di bidang pengurutan gen, teknologi pengurutan nanopore memicu revolusi teknologi dengan keunggulan uniknya. Sebagai teknologi pengurutan gen generasi keempat, it leverages the principles of single-molecule sequencing and innovative electrical signal detection, bringing unprecedented possibilities to life science research and clinical applications.

SAYA. The Principle of Nanopore Sequencing: The Genetic Code in Electric Currents

The core of nanopore sequencing lies in combining single-molecule detection with electrical signal transmission. Unlike traditional sequencing methods, nanopore sequencing does not require complex PCR amplification or rely on optical signals. Alih-alih, it directly reads DNA or RNA sequences through changes in electrical signals.

Specifically, nanopore sequencing utilizes a special nanopore protein that can be embedded into a high-resistance polymer film, forming an ionic channel. When a voltage is applied across the film, ions flow through the nanopore, generating an electrical current. The DNA or RNA molecules to be sequenced are then drawn through the nanopore in single-strand form by a motor protein. As different bases (A, T, C, G) pass through the nanopore, they cause varying degrees of current disruption. The sequencer records these fluctuations in electrical signals to deduce the order of the bases.

This electrical signal-based sequencing method not only simplifies sample processing but also significantly enhances sequencing efficiency. Misalnya, nanopore sequencing can achieve a sequencing speed of up to 450 bases per second per pore. Selain itu, nanopore sequencing boasts much longer read lengths compared to traditional technologies, with the longest reads reaching up to 4.2 Mb. This makes it highly advantageous for assembling complex genomes and discovering unknown structural variations.

II. The Advantages of Nanopore Sequencing: The Perfect Combination of Long Reads and Rapid Detection

Compared to traditional second-generation sequencing technologies (NGS), nanopore sequencing has made a leap from short reads to ultra-long reads. The advantage of long reads is their ability to more accurately span complex regions of the genome, such as highly repetitive sequences and structural variation regions, thereby improving the accuracy and completeness of sequencing.

Lebih-lebih lagi, the rapid detection capability of nanopore sequencing also makes it shine in clinical applications. Misalnya, in pathogen detection, nanopore sequencing can complete whole-genome sequencing of pathogens in a short time, quickly identifying the type of pathogen and its resistance genes, providing important evidence for clinical diagnosis and treatment.

AKU AKU AKU. Applications of Nanopore Sequencing: Broad Expansion from Laboratory to Clinical Settings

The application scope of nanopore sequencing is very broad, covering multiple fields including genomics, transkriptomik, epigenetics, dan deteksi patogen. In genomics research, it helps scientists more efficiently assemble complex genomes and reveal their structure and function. In pathogen detection, nanopore sequencing can rapidly identify infectious pathogens, including some that are difficult to detect with traditional methods.

Selain itu, nanopore sequencing can directly detect modifications of DNA and RNA, seperti metilasi. This is of great significance for studying gene expression regulation and disease mechanisms.

With its unique principles and powerful functions, nanopore sequencing technology is becoming an indispensable tool in life science research and clinical diagnostics. As the technology continues to advance and its applications expand, nanopore sequencing will bring more surprises and breakthroughs to human health and life science research.

Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 yang merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami .