Manik Magnetik Lnjnbio untuk Kit Ekstraksi Cepat DNA Plasmid 160866

(Metode Manik Magnetik)

【Pengenalan Produk】

This kit uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only requires several simple cleaning to remove endotoxin, protein, polysaccharide and other impurities, to obtain high quality plasmid DNA. The extraction of purified plasmid DNA from this product caused little damage to the plasmid, high extraction concentration and high supercoiled ratio. The extracted plasmid DNA can be used in application scenarios including enzyme digestion, PCR, pengurutan, ligation and transformation.

【Keunggulan produk】

1, The core raw materials are produced by ourselves, product quality and yield are guaranteed.

2, It can effectively remove endotoxin and improve transfection efficiency.

3, Simple and quick operation with no need for repeated centrifugation.

4, Aman dan tidak beracun: no need for organic reagents such as phenol/chloroform.

5, Kemurnian tinggi dari produk yang diekstraksi, hasil tinggi, high ratio of super-helix.

6, It can be applied to automation and high throughput operation.

【Gambar produk】

【Kasus eksperimental】

Kasus 1: Low-copy plasmid was cultured in 2 mL and collected by centrifugation.

The extraction results are as follows:

Nomor	A260/A280	A260/A230	Kesimpulan(ng/μL)
1	1.987	1.889	293.35
2	1.987	1.889	236.25
3	1.993	1.900	202.90
4	1.976	1.904	259.70
CV	0.3%	0.4%

Elektroforogram ditunjukkan di bawah ini:

Analisis hasil:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:：

① CV values were below 1%, demonstrating high reagent extraction stability.

② A260 / A280 range from 1.976 ke 1.993, and the values of A260 / A230 range from 1.889 ke 1.904. The peak shape is complete and the basic coincidence shows the high purity of the extraction product.

③ 2 mL culture solution can reach more than 20 µ g.

The results of the agarose gel electrophoresis then showed that:

① Four samples were selected, all with bright bands and good integrity.

Kesimpulannya, the plasmid DNA rapid extraction reagent was extracted from four bacterial liquid samples, the extraction product had high purity, hasil tinggi, complete electrophoretic strip, and the reagent and the instrument were stable.

Kasus 2: Low-copy plasmid was cultured in 10 mL and collected by centrifugation. The extraction results are as follows:

Nomor	A260/A280	A260/A230	Kesimpulan(ng/μL)
1	1.997	2.139	1538.35

Elektroforogram ditunjukkan di bawah ini:

Analisis hasil:

The test results of the ultra-trace nucleic acid tester show that:

① A260 / A280 = 1.997 and A260 / A230 = 2.139, menunjukkan kemurnian tinggi dari produk yang diekstraksi.

② Plasmid extraction yields of more than 150 µg can be achieved with 10mL of culture broth.

The results of the Ultra-Micro Nucleic Acid Assay test showed that:：

① The extracted product ran electrophoresis with bright bands and good integrity.

Kesimpulannya, 10ml of bacterial solution sample was extracted from plasmid DNA, and the extraction products had high purity, hasil tinggi dan pita elektroforesis lengkap.

【Aarea aplikasi】

The plasmid DNA extracted using this kit can be used in various biological experiments, seperti pencernaan enzim, pengurutan, library screening, ligation and transformation, dll..

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.