Manik Magnetik Lnjnbio untuk Kit Ekstraksi RNA Total Tanaman 160933

(Metode Manik Magnetik) 

【Pengenalan Produk】

Plant Total RNA Extraction Reagent is suitable for fast and efficient extraction of total RNA from plant tissue samples such as fresh plant leaves, fruits and seeds, dll.. The whole process is easy to operate, sangat cocok untuk platform ekstraksi asam nukleat otomatis. During the extraction process, the sample releases nucleic acid under the action of lysis solution, and the nano-magnetic beads selectively adsorb the nucleic acid under the action of binding solution, separating the nucleic acid from other impurities in the sample; and then rinsing with washing solution to remove proteins, polisakarida, ion garam anorganik, dll.. Akhirnya, high quality RNA can be obtained by elution, which is no need for toxic reagents such as phenol, khloroform, dll., and can be applied to various kinds of downstream molecules and can be applied to various kinds of molecules. It can be applied to various downstream molecular biology experiments.

【Keunggulan produk】

1, Mudah dioperasikan, magnetic bead method can be used with automatic nucleic acid extractor to realize automated extraction, lysis combined with one step, efficient extraction.

2, Without repeated centrifugation, to ensure the integrity of the extracted RNA.

3, Extracted products have good purity, hasil tinggi dan pita elektroforesis lengkap.

4, The use of process safety, tanpa penggilingan nitrogen cair, produk tidak mengandung fenol, kloroform dan reagen berbahaya lainnya.

5, Fleksibilitas yang kuat, suitable for extracting RNA from leaves, buah-buahan, seeds and other samples of different kinds of plants.
【Gambar produk】

 

【Kasus eksperimental】

Kasus 1:Fresh tender E.aureum leaves were taken for total RNA extraction experiments, dan hasilnya adalah sebagai berikut:

TIDAK. nomor	Asupan sampel（mg）	A260/A280	A260/A230	Kesimpulan(ng/µL)
1	50 mg	2.087	1.579	93.85
2		2.056	1.582	103.45
3		2.099	1.477	83.6
4		2.078	1.511	85.2

Elektroforogram ditunjukkan di bawah ini:

 

Analisis hasil:

The RNA product of E.aureum extracted with this product has high yield, complete bands and stable quality.

Kasus 2:Leaf samples of different plants were taken and total RNA was extracted with the following results:

TIDAK.	Mencicipi	A260/A280	A260/A230	Kesimpulan(ng/µL)	Hasil（µg）
1	20mg Daun ubi jalar	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Rata-rata		1.974	2.0385	194.1	19.41
3	20mg Daun persik	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Rata-rata		1.9025	1.4745	159.36	15.936
5	20mg Daun musiman	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Rata-rata		1.8565	1.7	99.06	9.906
7	20mg Daun terong	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Rata-rata		1.9875	1.7535	98.34	9.834

Diagram pola puncak:

Daun ubi jalar:

Peach leaves:

Seasonal leaves:

Eggplant leaves:

Diagram puncak total：

Elektroforogram ditunjukkan di bawah ini:

 

Analisis hasil:

Hasil Uji Asam Nukleat Ultra Mikro (UMNA) tes menunjukkan hal itu:

①The values of A260/A280 were in the range of 1.8~2.0, dan nilai A260/A230 berada di atas 1.4, and the peak shapes were complete and basically overlapped, yang membuktikan bahwa produk yang diekstraksi memiliki kemurnian tinggi.

②The yields of nucleic acids extracted from the leaves of different plants were above 9 mg.

Hasil elektroforesis gel agarosa menunjukkan bahwa:

① The samples of different plant leaves have bright bands and good integrity.

Kesimpulannya, produk yang diekstraksi dari sampel daun tanaman yang berbeda memiliki kemurnian tinggi, hasil tinggi dan pita elektroforesis lengkap.

Kasus 3:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

TIDAK.	Mencicipi	A260/A280	A260/A230	Kesimpulan(ng/µL)	Hasil（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Rata-rata		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Rata-rata		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Rata-rata		1.955	2.23	219.78	21.978

Diagram pola puncak:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Diagram puncak total:

 

Elektroforogram ditunjukkan di bawah ini:

 

Analisis hasil:

Hasil Uji Asam Nukleat Ultra Mikro (UMNA) tes menunjukkan hal itu:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, dan nilai A260/A230 berada di atas 1.6, and the peak shapes were complete and basically overlapped, yang membuktikan bahwa produk yang diekstraksi memiliki kemurnian tinggi.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 mg.

Hasil elektroforesis gel agarosa menunjukkan bahwa:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

Kesimpulannya, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, hasil tinggi dan pita elektroforesis lengkap.

【Aarea aplikasi】

The extracted and purified nucleic acids from this kit can be used in a variety of downstream experiments such as RT-PCR, PCR Waktu Nyata, microarray analysis, penyaringan poliA, terjemahan in vitro dan kloning molekuler.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami di sales01@lingjunbio.com.