Lnjnbio Magnetic Beads for Plant Total RNA Extraction Kit 160933

(Метод магнитных шариков) 

【Представление продукта】

Plant Total RNA Extraction Reagent is suitable for fast and efficient extraction of total RNA from plant tissue samples such as fresh plant leaves, fruits and seeds, и т. д.. The whole process is easy to operate, especially suitable for automated nucleic acid extraction platform. During the extraction process, the sample releases nucleic acid under the action of lysis solution, and the nano-magnetic beads selectively adsorb the nucleic acid under the action of binding solution, separating the nucleic acid from other impurities in the sample; and then rinsing with washing solution to remove proteins, polysaccharides, inorganic salt ions, и т. д.. Окончательно, high quality RNA can be obtained by elution, which is no need for toxic reagents such as phenol, chloroform, и т. д., and can be applied to various kinds of downstream molecules and can be applied to various kinds of molecules. It can be applied to various downstream molecular biology experiments.

【Преимущества продукта】

1, Easy to operate, magnetic bead method can be used with automatic nucleic acid extractor to realize automated extraction, lysis combined with one step, efficient extraction.

2, Without repeated centrifugation, to ensure the integrity of the extracted RNA.

3, Extracted products have good purity, высокий выход и полные электрофоретические полосы.

4, The use of process safety, without liquid nitrogen grinding, продукт не содержит фенол, хлороформ и другие вредные реагенты.

5, Сильная универсальность, suitable for extracting RNA from leaves, fruits, seeds and other samples of different kinds of plants.
【Изображение продукта】

 

【Экспериментальный случай】

Случай 1:Fresh tender E.aureum leaves were taken for total RNA extraction experiments, and the results were as follows:

Нет. number	Sample intake（mg）	А260/А280	А260/А230	Конц.(ng/µL)
1	50 mg	2.087	1.579	93.85
2		2.056	1.582	103.45
3		2.099	1.477	83.6
4		2.078	1.511	85.2

The electropherogram is shown below:

 

Анализ результатов:

The RNA product of E.aureum extracted with this product has high yield, complete bands and stable quality.

Случай 2:Leaf samples of different plants were taken and total RNA was extracted with the following results:

No.	Образец	А260/А280	А260/А230	Конц.(ng/µL)	Yield（µg）
1	20mg Sweet potato leaves	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Avg		1.974	2.0385	194.1	19.41
3	20mg Peach leaves	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Avg		1.9025	1.4745	159.36	15.936
5	20mg Seasonal leaves	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Avg		1.8565	1.7	99.06	9.906
7	20mg Eggplant leaves	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Avg		1.9875	1.7535	98.34	9.834

Peak pattern diagram:

Sweet potato leaves:

Peach leaves:

Seasonal leaves:

Eggplant leaves:

Total peak diagram：

The electropherogram is shown below:

 

Анализ результатов:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①The values of A260/A280 were in the range of 1.8~2.0, and the values of A260/A230 were above 1.4, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 9 µg.

The results of agarose gel electrophoresis showed that:

① The samples of different plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different plant leaves samples were of high purity, высокий выход и полные электрофоретические полосы.

Случай 3:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

No.	Образец	А260/А280	А260/А230	Конц.(ng/µL)	Yield（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Avg		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Avg		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Avg		1.955	2.23	219.78	21.978

Peak pattern diagram:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Total peak diagram:

 

The electropherogram is shown below:

 

Анализ результатов:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, and the values of A260/A230 were above 1.6, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 µg.

The results of agarose gel electrophoresis showed that:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, высокий выход и полные электрофоретические полосы.

【Аобласть применения】

The extracted and purified nucleic acids from this kit can be used in a variety of downstream experiments such as RT-PCR, Real-Time PCR, microarray analysis, polyA screening, in vitro translation and molecular cloning.

Введение поставщика

Шанхайская биотехнологическая компания Линцзюнь., ОООбыл создан в 2016 и является профессиональным производителем биомагнитных материалов и реагентов для экстракции нуклеиновых кислот..

Мы имеем богатый опыт в экстракции и очистке нуклеиновых кислот., очистка белка, разделение клеток, хемилюминесценция и другие области техники.

Наша продукция широко используется во многих областях., например, медицинское тестирование, генетическое тестирование, университетские исследования, генетическое разведение, и так далее. Мы не только поставляем продукцию, но также можем взять на себя OEM, ОДМ, и другие потребности. Если у вас есть схожие потребности, пожалуйста, не стесняйтесь обращаться к нам по адресу sales01@lingjunbio.com.