血液サンプル処理を最適化し、RNA 抽出の品質を確保します。

生物医学研究において, high-quality RNA extraction is crucial to ensure the accuracy of experimental results. 一般的に使用される生体サンプルとして, every detail in the processing of blood samples will directly affect the quality of the final RNA. To reduce the risk of RNA degradation, we will explore some effective blood sample pretreatment.

私. Fast and efficient sample processing

Blood samples should be centrifuged as soon as possible after collection to avoid hemolysis caused by the rupture of red blood cells. Hemolysis not only releases hemoglobin, but also activates endogenous RNase, thereby accelerating RNA degradation. したがって, it is recommended to complete centrifugation and aliquoting within 3-5 hours after sample collection.

Ⅱ. Choose the right blood collection tube

Using blood collection tubes specifically designed for RNA preservation, such as PAXgene whole blood RNA blood collection tubes, can protect RNA from degradation to a certain extent. These blood collection tubes have built-in protective agents that can effectively inhibit RNase activity and ensure the stability of RNA.

Ⅲ. Add protective agent

If the sample cannot be processed immediately, consider adding a protective agent such as RNAlater or TRIzol to the sample. These reagents can further inhibit RNase activity and protect RNA from environmental factors.

The quality of RNA in blood samples can be significantly improved through the above measures and provide a reliable basis for subsequent research.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

当社は核酸の分離・精製において豊富な経験を持っています, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

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