Optimize the blood sample processing and ensure the RNA extraction quality

In biomedical research, high-quality RNA extraction is crucial to ensure the accuracy of experimental results. As a commonly used biological sample, every detail in the processing of blood samples will directly affect the quality of the final RNA. To reduce the risk of RNA degradation, we will explore some effective blood sample pretreatment.

RNA structure, RNA preparation, RNA isolation, RNA preservation — RNA structure

I. Fast and efficient sample processing

Blood samples should be centrifuged as soon as possible after collection to avoid hemolysis caused by the rupture of red blood cells. Hemolysis not only releases hemoglobin, but also activates endogenous RNase, thereby accelerating RNA degradation. Therefore, it is recommended to complete centrifugation and aliquoting within 3-5 hours after sample collection.

II. Choose the right blood collection tube

Using blood collection tubes specifically designed for RNA preservation, such as PAXgene whole blood RNA blood collection tubes, can protect RNA from degradation to a certain extent. These blood collection tubes have built-in protective agents that can effectively inhibit RNase activity and ensure the stability of RNA.

Blood collection tube, RNA preparation, RNA isolation, RNA preservation — Blood collection tubes

III. Add protective agent

If the sample cannot be processed immediately, consider adding a protective agent such as RNAlater or TRIzol to the sample. These reagents can further inhibit RNase activity and protect RNA from environmental factors.

The quality of RNA in blood samples can be significantly improved through the above measures and provide a reliable basis for subsequent research.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid isolation and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, etc. We not only provide products but also can undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.