Shanghai LNJNBio nucleic acid extraction program is escorting the detection of African swine fever

At a time when the prevention and control of African swine fever (ASF) remains a serious challenge, rapid and accurate laboratory testing serves as the first line of defense for early detection and timely response to outbreaks. The accuracy and reliability of test results largely depend on the quality of nucleic acid (DNA and RNA) extraction from samples.

In ASFV (African Swine Fever Virus) detection, complex scenarios such as viral mutations and mixed infections (including co-infection with classical swine fever CSFV requiring simultaneous RNA virus testing) have emerged. Traditional single-nucleic acid extraction methods now face challenges including cumbersome procedures, high sample consumption, and cross-contamination risks, making them inadequate for the urgent demands of high-throughput multi-target integrated testing.

Shanghai Lingjun Biotechnology Co., Ltd. has been deeply engaged in the field of molecular diagnostic core raw materials and reagents. With profound research and development strength, it has launched an animal tissue DNA/RNA co-extraction kit, providing a new “integrated” solution for ASF detection.

Why choose co-extraction? The pain point and innovation of ASF detection

Addressing viral diversity: While African swine fever virus (ASFV) is a DNA virus, its diagnosis often requires differentiation from CSF viruses (RNA viruses) during epidemic control. The co-treatment technology enables simultaneous extraction of high-quality DNA and RNA from a single sample in one operation, eliminating the need for two separate procedures. This approach conserves valuable samples and time for multi-pathogen joint detection.

Enhanced detection sensitivity: Particularly suitable for late-stage infections or tissue samples with severe nucleic acid degradation, where viral loads may be low. Lingjun Bio’s co-treatment kit utilizes a unique lysis buffer and buffer system that efficiently releases and protects intact nucleic acid molecules, maximizing detection efficiency.

Ultimate Efficiency and Cost-effectiveness: By combining two extraction steps into one, this kit significantly reduces operational steps, saves consumables (such as pipettes and centrifuge tubes), and minimizes cross-contamination risks from repeated lid openings – making it ideal for large-scale sample screening.

User-Friendly and Reliable: The optimized process eliminates toxic phenol/chloroform, ensuring safe and efficient operation. The entire workflow can be completed within 40-60 minutes, yielding high-purity nucleic acids with excellent integrity that are compatible with various downstream molecular detection platforms including qPCR, RT-qPCR, and digital PCR.

 Core Advantages of Lingjun Biotech’s Co-extraction Kit: High Yield and Purity: Specifically optimized for animal tissues (e.g., lymph nodes, spleen, liver, blood), effectively overcoming interference from polysaccharides, lipids, and hemoglobin inhibitors in tissues. The ideal A260/A280 ratio enables direct use in highly sensitive qPCR experiments.

Exceptional Compatibility: Extracted nucleic acids are applicable not only for ASFV detection but also for other swine pathogens (e.g., PRRSV, PCV2, CSFV), truly achieving “one extraction, multiple detections”.

 Powerful Endogenous RNase Inhibition: Contains high-efficiency RNase inhibitors to ensure RNA degradation during extraction, guaranteeing accurate viral detection results.

 High Batch Stability: Strict production quality control ensures consistent performance across batches, maintaining reliable and comparable laboratory results.

Experimental cases ：

Different tissue samples of different animals, DNA/RNA extraction

Number	Sample	A260/A280	A260/A230	Conc.(ng/μL)	Yield(μg)
1	20mgFish	1.86	1.88	109.25	10.93
2		1.87	1.96	117.40	11.74
Average		1.86	1.92	113.33	11.33
3	20mg Chicken meat	1.89	2.05	284.55	28.46
4		1.89	2.04	249.40	24.94
Average		1.89	2.04	266.98	26.70
5	5mg Fish liver	1.81	1.59	256.45	25.65
6		1.81	1.57	253.40	25.34
Average		1.81	1.58	254.93	25.49
7	5mg Chicken heart	1.86	1.97	101.95	10.20
8		1.85	2.00	107.20	10.72
Average		1.85	1.98	104.58	10.46

 Agarose gel electrophoresis ：

Results Analysis:

The A260/A280 ratio exceeded 1.8, while the A260/A230 ratio ranged between 1.5-2.0. Nucleic acid extraction yields from different animal tissue samples ranged from 10-28 µg. Bright bands with good integrity were observed across various animal tissues and samples.

In summary, the fully automated nucleic acid extraction and purification system demonstrates high purity, high yield, intact electrophoretic bands, and stable reagent-instrument compatibility when processing nucleic acids from different animal samples.

Application Scenarios:

Routine ASF monitoring and epidemic investigation at CDCs; Risk monitoring of imported pork and products by customs and port quarantine authorities; Biosafety monitoring and biosecurity assessments for large-scale pig farming groups and food processing enterprises; Pathogen-related epidemiological research in universities and research institutes.

Shanghai Lingjun Biotechnology drives innovation while prioritizing quality. Our animal tissue DNA/RNA co-extraction kit serves as a powerful tool to enhance diagnostic capabilities and strengthen ASF prevention in veterinary laboratories. Let us collaborate through advanced technologies and reliable products to safeguard the healthy development of the hog industry!

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us .