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magnetic bead method
How To Extract Nucleic Acid By Magnetic Bead Method
For first-time users of magnetic bead nucleic acid extraction kits, paying close attention to the key details of handling the magnetic beads, in addition to other routine procedures, will greatly increase the…
What are the advantages of magnetic bead method for RNA extraction compared with Trizol method?
1.To understand the differences between the two methods, it is essential to first grasp their principles: The Trizol method involves using organic solvents like phenol and chloroform to lyse cells, thereby separating…
What should be paid attention to in the magnetic bead method of blood RNA extraction?
Precautions for Magnetic Bead-Based Blood RNA Extraction 1. Sample Collection and Handling 1.1 Anticoagulant Selection Preferred: EDTA,inhibits calcium-dependent nucleases. Acceptable: ACD Avoid: Heparin (inhibits downstream applications) 1.2 Processing Timeline Process samples immediately after collection…
How to improve the results of the 260/230 calculation using the magnetic bead method for extracting reagents?
When extracting nucleic acids using the magnetic bead method, a low 260/230 ratio (usually < 1.8) indicates the presence of organic compounds (such as guanidine salts, phenol, ethanol, EDTA) or residual salts in…
Market research report of nucleic acid extraction kit by magnetic bead method
1. Industry background and development status quo As a core tool in the field of molecular diagnostics and biotechnology, the magnetic bead nucleic acid extraction kit has expanded rapidly in recent years…
Nucleic Acid Extraction by Magnetic Bead Method:Revealing the Components and Functions of Reagents
The components and functions of various reagents for extracting DNA by the magnetic bead method are as follows. 1. Lysis Buffer (1) SDS (Sodium Dodecyl Sulfate): An anionic surfactant that disrupts cell membrane structures, releasing cellular contents. It can break…
Ensuring the quality of vermicelli-easily detect cassava genes with magnetic bead method
New Year’s shopping becomes the top priority for every household as the New Year comes. Vermicelli is undoubtedly an indispensable dish on the Spring Festival table among the myriad of traditional delicacies. However, how to ensure the…
Basic strategies and methods for de-endotoxinization during plasmid DNA purification
The use of endotoxin-free plasmids should be considered when the extracted plasmid DNA is used for transfection of primary, suspension, or sensitive cells, gene silencing studies, cell injection micromanipulation, gene therapy or…
A brief analysis of the principle of extracting small DNA fragments by the magnetic bead method
small DNA fragments、magnetic bead method
Shanghai Lingjun Biotechnology Co., Ltd. Passed the ISO9001 and ISO13485 Annual Renewal Review Successfully
On September 13, 2024, Shanghai Lingjun Biotechnology Co., Ltd. completed the annual renewal review of ISO 9001 and ISO 13485 quality management systems successfully. The expert team sent by the Shanghai Yinggeer…
Common Problems in Magnetic Bead Method Nucleic Acid Extraction
In the process of nucleic acid detection, magnetic bead extraction is widely used because of its high detection sensitivity, automatizable operation, high throughput, safety, and rapidity. However, in the process of nucleic…