Application analysis of alcohol-free nucleic acid extraction reagent

Main application areas of alcohol-free extraction reagents:

1. Virus nucleic acid extraction

In infectious disease detection (such as novel coronavirus and influenza virus), alcohol-free reagents can rapidly extract viral nucleic acids by magnetic bead method. For example, Big Fly’s patented technology achieves efficient lysis and purification through one-step washing, and the sensitivity is improved to detect viral RNA as low as 10 copies.

2. Blood sample processing

The extraction of nucleic acids from blood requires extremely high purity. The alcohol-free reagent significantly improves the yield of DNA/RNA by optimizing the combination of lysis buffer (containing guanidinium hydrochloride, Triton X-100, etc.) and washing buffer (containing PEG-8000). Comparative experiments show that its Ct value is 2 units lower than traditional methods, with a recovery efficiency four times higher.

3. Diversified sample compatibility

In addition to blood and virus samples, alcohol-free reagents can also process a variety of sample types such as throat swabs, nasal swabs, serum, plasma, etc., and are suitable for the differentiated processing process of dry/wet swabs.

4. Future development direction

Technology optimization: Further development of multi-parameter detection kits (such as simultaneous DNA and RNA extraction) and optimization of the automated washing procedure through AI algorithms.

Market expansion: Promote the application of alcohol-free reagents in primary medical care and home self-examination, such as portable POCT equipment with reagents.

Unified industry standards: At present, there are differences in data interfaces and testing standards of different brands of reagents, so it is necessary to establish unified norms through policy guidance.

Alcohol-free extraction reagents are gradually replacing traditional alcohol-containing solutions due to their safety, high efficiency and environmental protection, and have become an important technical support in molecular diagnosis, industrial detection and other fields. With the iteration of technology and the expansion of market demand, its application prospect will be further expanded.

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us at sales01@lingjunbio.com.