التقييم العملي لمجموعة أدوات استخراج الحمض النووي الريبي (RNA) الشاملة ذات الأغراض العامة والمبنية على الخرز المغناطيسي

As we know, استخراج الحمض النووي الريبي is a basic procedure, the results are often fraught with uncertainty. This is particularly true of animal tissue samples: different tissues—such as liver, muscle, brain and spleen—vary significantly in texture, endogenous enzyme content and fat content, and the slightest misstep can lead to RNA degradation or insufficient purity. Many laboratories are forced to stock multiple kits for different tissues, which not only takes up space but also leads to confusion; should the extraction of a precious sample fail, it can seriously disrupt the progress of the experiment.

اليوم, we carried out RNA extraction from animal tissue using the newly launched magnetic bead-Based universal total RNA extraction kit from Shanghai Lingjun Biotechnology. The instrument used was the LJbio32H automated nucleic acid extraction and purification system. النتائج التجريبية هي كما يلي:

Agarose gel electrophoresis（1.5% 120V 25min）：

من حيث الطهارة, yield and the integrity of the RNA bands, this kit delivers excellent extraction results, making it fully capable of meeting customers’ requirements for demanding downstream experiments such as cDNA library construction, qPCR, Northern blotting and RNA-seq.

The core of this kit lies in its unique lysis system and magnetic bead purification technology. The entire process requires no phenol/chloroform extraction; instead, it relies on an optimised lysis buffer to rapidly disrupt a variety of animal tissues—including liver, muscle, brain, heart and spleen—thereby fully releasing RNA whilst effectively inhibiting RNase activity. Magnetic beads specifically designed to bind nucleic acids are then added; under specific buffer conditions, the RNA binds efficiently to the surface of the magnetic beads. Solid-liquid separation is easily achieved by applying a magnetic field via a magnetic stand. After a simple washing step to remove impurities such as proteins and lipids, the RNA is eluted using enzyme-free water to yield high-purity total RNA. The entire manual procedure is straightforward and takes approximately 25–30 minutes. The kit’s protocol can also be easily adapted for use with various automated nucleic acid extraction systems, enabling standardised extraction at higher throughput.

The product incorporates a highly efficient genomic DNA removal step, which significantly reduces gDNA residue, ensuring clearer and more reliable negative control results in downstream RT-qPCR. The instruction manual also thoughtfully provides recommended tissue quantities and lysis times tailored to the characteristics of different animal tissues, enabling users to get started smoothly even when processing fibrous muscle tissue or fatty brain tissue for the first time.

In practical terms, this universal magnetic bead-based RNA extraction kit from Shanghai Lingjun is suitable for a wide range of animal tissue samples, including liver, muscle, brain, قلب, الطحال, lung and kidney, truly offering the convenience of requiring just one flagship kit per laboratory.

At a time when reagent supply is often subject to fluctuations, Shanghai Lingjun Biotechnology, as a leading domestic supplier of high-quality nucleic acid extraction reagents, offers this magnetic bead-based universal RNA extraction kit, which not only delivers outstanding performance but also boasts impressive delivery speed and value for money. بالإضافة إلى, a dedicated technical support team is on hand to resolve any issues that may arise during the extraction process.

مزود

شركة شنغهاي لينغجون للتكنولوجيا الحيوية, المحدودة.تأسست في 2016 وهي شركة متخصصة في تصنيع المواد المغناطيسية الحيوية وكواشف استخلاص الحمض النووي.

لدينا خبرة غنية في استخراج وتنقية الحمض النووي, تنقية البروتين, فصل الخلايا, التألق الكيميائي, وغيرها من المجالات التقنية.

منتجاتنا تستخدم على نطاق واسع في العديد من المجالات, مثل الفحوصات الطبية, الاختبارات الجينية, البحوث الجامعية, التكاثر الوراثي, وهكذا. نحن لا نقدم المنتجات فحسب، بل يمكننا أيضًا إجراء تصنيع المعدات الأصلية, أوديإم, وغيرها من الاحتياجات. إذا كان لديك حاجة ذات صلة, لا تتردد في الاتصال بنا .