Praktisk evaluering af et universelt, magnetisk perle-baseret universelt total-RNA-ekstraktionssæt

As we know, RNA-ekstraktion is a basic procedure, the results are often fraught with uncertainty. This is particularly true of animal tissue samples: different tissues—such as liver, muskel, brain and spleen—vary significantly in texture, endogenous enzyme content and fat content, and the slightest misstep can lead to RNA degradation or insufficient purity. Many laboratories are forced to stock multiple kits for different tissues, which not only takes up space but also leads to confusion; should the extraction of a precious sample fail, it can seriously disrupt the progress of the experiment.

I dag, we carried out RNA extraction from animal tissue using the newly launched magnetic bead-Based universal total RNA extraction kit from Shanghai Lingjun Biotechnology. The instrument used was the LJbio32H automated nucleic acid extraction and purification system. De eksperimentelle resultater er som følger:

Agarose gel electrophoresis（1.5% 120V 25min）：

Med hensyn til renhed, yield and the integrity of the RNA bands, this kit delivers excellent extraction results, making it fully capable of meeting customers’ requirements for demanding downstream experiments such as cDNA library construction, qPCR, Northern blotting and RNA-seq.

The core of this kit lies in its unique lysis system and magnetic bead purification technology. The entire process requires no phenol/chloroform extraction; instead, it relies on an optimised lysis buffer to rapidly disrupt a variety of animal tissues—including liver, muskel, hjerne, heart and spleen—thereby fully releasing RNA whilst effectively inhibiting RNase activity. Magnetic beads specifically designed to bind nucleic acids are then added; under specifikke bufferbetingelser, the RNA binds efficiently to the surface of the magnetic beads. Solid-liquid separation is easily achieved by applying a magnetic field via a magnetic stand. After a simple washing step to remove impurities such as proteins and lipids, the RNA is eluted using enzyme-free water to yield high-purity total RNA. The entire manual procedure is straightforward and takes approximately 25–30 minutes. The kit’s protocol can also be easily adapted for use with various automated nucleic acid extraction systems, enabling standardised extraction at higher throughput.

The product incorporates a highly efficient genomic DNA removal step, which significantly reduces gDNA residue, ensuring clearer and more reliable negative control results in downstream RT-qPCR. The instruction manual also thoughtfully provides recommended tissue quantities and lysis times tailored to the characteristics of different animal tissues, enabling users to get started smoothly even when processing fibrous muscle tissue or fatty brain tissue for the first time.

In practical terms, this universal magnetic bead-based RNA extraction kit from Shanghai Lingjun is suitable for a wide range of animal tissue samples, including liver, muskel, hjerne, hjerte, milt, lung and kidney, truly offering the convenience of requiring just one flagship kit per laboratory.

At a time when reagent supply is often subject to fluctuations, Shanghai Lingjun Biotechnology, as a leading domestic supplier of high-quality nucleic acid extraction reagents, offers this magnetic bead-based universal RNA extraction kit, which not only delivers outstanding performance but also boasts impressive delivery speed and value for money. Desuden, a dedicated technical support team is on hand to resolve any issues that may arise during the extraction process.

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens, og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har et relateret behov, er du velkommen til at kontakte os .