Magnetisk perlebaseret nukleinsyreekstraktionsteknologi: Fører effektivitetsrevolutionen i den biofarmaceutiske industri

In the current era of surging demands for precision medicine and large-scale disease screening, a key technology is quietly driving the efficiency revolution in the biopharmaceutical industry – magnetisk perle-baseret nukleinsyreekstraktion. With its significant advantages of high efficiency, automatisering, and high purity, this technology has rapidly become an indispensable core tool in molecular diagnostics, gensekventering, and new drug development fields.

Using our serum/plasma virus nucleic acid extraction reagents, HBV national standard gradient diluents were extracted on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of each concentration gradient diluent were detected by HBV-qPCR, with a correlation coefficient R>0.99. The specific data is as follows:

Resultatanalyse：The accuracy of the reagent is very high, and the sensitivity is still good when extracting low concentration samples.

Compared with the traditional centrifugal column method, magnetic bead-based extraction achieves rapid separation and purification through the specific binding of nucleic acids to surface-modified magnetic particles under the action of an external magnetic field. This principle enables breakthroughs in throughput, hastighed, and consistency. I de senere år, this technology has excelled in scenarios such as large-scale screening of infectious diseases (such as COVID-19 nucleic acid testing), tumor liquid biopsy (microscopic ctDNA capture), and genetic disease analysis, effectively supporting high-throughput, standardized modern testing requirements.

Nucleic acids were extracted from trace amounts of human Hela cells as samples, 5 celler, 10 celler, 20 celler, 500 cells were extracted, and the nucleic acids obtained were analyzed using Agilent 4200 Nucleic acid fragment distribution, and the results obtained are shown below: 

1. The Electropherogram of Agilent 4200 High Sensitivity RNA ScreenTape Assay

D1: 50 Hela cellsG1: 20 Hela cellsB2: 10 Hela cellsE2: 5 Hela cells

2. Peak diagram of the 50/20/10/5 Hela cells extracted

• Peak diagram of the 5 Hela cells extracted

• . Qubit’s test results

Ingen.	Prøveindtagelse	Udbytte（ng/μL）
1	50 Hela cells	0.242
2	20 Hela cells	0.136
3	10 Hela cells	0.112
4	5 Hela cells	0.096

Resultatanalyse： By Agilent 4200 electrophoresis results can be seen: 5– 50 traces of human Hela cell samples, can be obtained by the nucleic acid fragments, The extraction products were 18s RNA, 28sRNA, and DNA over 800bp.

The wave of industry innovation continues to surge. Domestic leading enterprises have launched fully automated solutions integrating magnetic bead extraction, opnår “sample input and result output” in closed tubes, reducing extraction time to minutes and significantly enhancing emergency testing capabilities. På samme tid, breakthroughs in domestic high-end magnetic bead raw materials, such as those from companies like Shanghai Lingjun Biotechnology, have broken the import monopoly, reducing the cost of a single sample by over 30%, significantly enhancing the autonomy of the industrial chain. Capital markets have shown great favor for this sector, with many related enterprises completing large-scale financing recently, driving technological iteration into a fast lane.

Ser på fremtiden, with the explosion of personalized medical care and immediate testing (POCT) demands, magnetic bead-based technology is evolving towards higher sensitivity, better anti-interference performance, and green biodegradability. Its combination with microfluidics and artificial intelligence is expected to give rise to a new generation of intelligent extraction platforms, continuously providing key infrastructure for the innovation and development of the biopharmaceutical industry.

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens, og andre tekniske områder.

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