Tecnologia di estrazione degli acidi nucleici basata su perline magnetiche: Alla guida della rivoluzione dell’efficienza nel settore biofarmaceutico

In the current era of surging demands for precision medicine and large-scale disease screening, a key technology is quietly driving the efficiency revolution in the biopharmaceutical industry – Estrazione dell'acido nucleico basata su perline magnetiche. With its significant advantages of high efficiency, automazione, ed elevata purezza, this technology has rapidly become an indispensable core tool in molecular diagnostics, sequenziamento dei geni, and new drug development fields.

Utilizzando i nostri reagenti per l'estrazione dell'acido nucleico del virus del siero/plasma, I diluenti del gradiente standard nazionale dell'HBV sono stati estratti sullo strumento automatico di estrazione e purificazione dell'acido nucleico LJbio32H. I prodotti estratti di ciascun diluente del gradiente di concentrazione sono stati rilevati mediante HBV-qPCR, con un coefficiente di correlazione R>0.99. I dati specifici sono i seguenti:

Analisi dei risultati：La precisione del reagente è molto elevata, e la sensibilità è ancora buona quando si estraggono campioni a bassa concentrazione.

Compared with the traditional centrifugal column method, magnetic bead-based extraction achieves rapid separation and purification through the specific binding of nucleic acids to surface-modified magnetic particles under the action of an external magnetic field. This principle enables breakthroughs in throughput, velocità, and consistency. Negli ultimi anni, this technology has excelled in scenarios such as large-scale screening of infectious diseases (such as COVID-19 nucleic acid testing), tumor liquid biopsy (microscopic ctDNA capture), and genetic disease analysis, effectively supporting high-throughput, standardized modern testing requirements.

Nucleic acids were extracted from trace amounts of human Hela cells as samples, 5 cellule, 10 cellule, 20 cellule, 500 cells were extracted, and the nucleic acids obtained were analyzed using Agilent 4200 Nucleic acid fragment distribution, and the results obtained are shown below: 

1. The Electropherogram of Agilent 4200 High Sensitivity RNA ScreenTape Assay

D1: 50 Hela cellsG1: 20 Hela cellsB2: 10 Hela cellsE2: 5 Hela cells

2. Peak diagram of the 50/20/10/5 Hela cells extracted

• Peak diagram of the 5 Hela cells extracted

• . Qubit’s test results

NO.	Assunzione del campione	Prodotto（ng/μL）
1	50 Hela cells	0.242
2	20 Hela cells	0.136
3	10 Hela cells	0.112
4	5 Hela cells	0.096

Analisi dei risultati： By Agilent 4200 electrophoresis results can be seen: 5– 50 traces of human Hela cell samples, can be obtained by the nucleic acid fragments, The extraction products were 18s RNA, 28sRNA, and DNA over 800bp.

The wave of industry innovation continues to surge. Domestic leading enterprises have launched fully automated solutions integrating magnetic bead extraction, raggiungimento “sample input and result output” in closed tubes, reducing extraction time to minutes and significantly enhancing emergency testing capabilities. Allo stesso tempo, breakthroughs in domestic high-end magnetic bead raw materials, such as those from companies like Shanghai Lingjun Biotechnology, have broken the import monopoly, reducing the cost of a single sample by over 30%, significantly enhancing the autonomy of the industrial chain. Capital markets have shown great favor for this sector, with many related enterprises completing large-scale financing recently, driving technological iteration into a fast lane.

Looking to the future, with the explosion of personalized medical care and immediate testing (POCT) demands, magnetic bead-based technology is evolving towards higher sensitivity, better anti-interference performance, and green biodegradability. Its combination with microfluidics and artificial intelligence is expected to give rise to a new generation of intelligent extraction platforms, continuously providing key infrastructure for the innovation and development of the biopharmaceutical industry.

Fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 che è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza, e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere l'OEM, ODM, e altre esigenze. Se hai un'esigenza correlata, non esitate a contattarci .