Eliminare i colli di bottiglia nell’estrazione: I kit RNA con perline magnetiche Lingjun ridefiniscono gli standard di efficienzaPotenziando la ricerca con soluzioni di acidi nucleici ad elevata purezza

Nella ricerca sulla biologia molecolare, L’estrazione dell’RNA da campioni complessi rimane un collo di bottiglia critico che ostacola l’efficienza sperimentale. I metodi tradizionali lottano con l’interferenza dei polisaccaridi/polifenoli vegetali, rischi di degradazione dei tessuti animali, and capturing trace viral nucleic acids—often being time-consuming while compromising purity and yield. With over 13 years of expertise in magnetic bead nucleic acid extraction, Shanghai Lingjun Biotechnology leverages core magnetic bead R&D capabilities to deliver a full-scene magnetic bead RNA extraction kit series, providing researchers with an efficient, sicuro, and stable one-stop solution.

IO. Why Choose Lingjun? Core Technologies Deliver Unmatched Advantages

In-House Magnetic Bead R&D: Industry-Leading Performance

Lingjun’s self-developed superparamagnetic silica beads(1.4μm particle size, 65±5 emu/g magnetic saturation) achieve >95% nucleic acid adsorption through surface modification and functional design. Batch-to-batch consistency eliminates material variability, ensuring reproducible results across laboratories.

“One-Step Protocol” Revolution: Boosts Efficiency by >50%

No centrifugation or toxic reagents: Eliminates hazardous phenol/chloroform and liquid nitrogen grinding. Room-temperature lysis with magnetic bead adsorption delivers pure RNA in under 1 ora.

Full automation compatibility: Kits adapt to mainstream nucleic acid extractors. Magnetic-rod platforms require no manual intervention, abilitante “carica e vai” operation.

3.Conquering Four Challenging Samples: Purity Meets Top Journal Standards

Sample Type	Key Innovation	Prestazione (A260/A280)
Polysaccharide/polyphenol plants	Proprietary lysis buffer removes impurities	1.90–2.0 (dandelion leaf)
Animal tissues (muscle/liver)	Grinding-free direct lysis, 100% RNA integrity	1.86–1.96 (shrimp/fish liver)
Serum/plasma viruses	Sensitivity down to 10 UI/ml, repeatability R>0.99	Full detection in gradient dilutions
Trace cells	Extraction from 0.2 mg, zero waste for rare samples	32.7 μg from 20 mg pork

II. Flagship Product Matrix: Precision Solutions for Research Scenarios

Plant RNA “Specialist” – Polysaccharide/Polyphenol Kit

Processes challenging samples (pothos, peach trees, roses) In 30 minuti. Pre-packaged kits (96 tests/box) yield high-purity RNA (A260/A230≥1.8), >9 μg/20 mg leaves—ideal for transcriptome sequencing.

Kit di estrazione dell'RNA totale vegetale

Caso sperimentale:

 20mg of sweet potato leaves, peach leaves, rose leaves and eggplant leaves were extracted with LNJNBio plant total RNA extraction kit

No.	Campione	A260/A280	A260/A230	Concentrato(ng/μL)	Yield（µg）
1	20mg Foglie di patata dolce	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Media		1.974	2.0385	194.1	19.41
3	20mg Peach leaves	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Media		1.9025	1.4745	159.36	15.936
5	20mg Seasonal leaves	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Media		1.8565	1.7	99.06	9.906
7	20mg Eggplant leaves	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Media		1.9875	1.7535	98.34	9.834

Gel electrophoresis with agarose:

Analisi dei risultati： 

The A260/A280 ratio was between 1.8-2.0, while the A260/A230 ratio exceeded 1.4, indicating high purity of the extracted products. All plant leaf samples achieved nucleic acid extraction yields above 9 mg. Bright and well-integrated bands were observed in all plant leaf samples. In sintesi, the extraction process yielded high-purity products with substantial output and complete electrophoretic bands when processing different plant leaf samples.

Animal RNA “Guardian” – Degradation-Proof Design

From shrimp to mouse tails, centrifugation-free technology ensures clear 28S/18S bands and 100% electrophoresis integrity. 50-test boxes support high-throughput labs.

Kit per l'estrazione dell'RNA totale da tessuti animali

Caso sperimentale:

The total RNA extraction kit of animal tissues was used to extract shrimp meat, chicken heart, fish heart, fish liver and fish gut respectively

NO.	Campione	A260/A280	A260/A230	Concentrato(di/ul)	Yield（μg）
1	20mg Gamberetti	1.863	1.725	65.48	5.2384
2		1.865	1.782	68.96	5.5168
Media		1.864	1.7535	67.22	5.3776
3	5mg Cuore di pollo	1.958	1.721	55.72	0.44576
4		1.966	1.736	56.68	0.45344
Media		1.962	1.7285	56.2	0.4496
5	5mg Cuore di pesce	1.944	1.761	28.56	0.22848
6		1.946	1.742	27.84	0.22272
Media		1.945	1.7515	28.2	0.2256
7	5mg Fegato di pesce	1.911	2.07	91.84	0.73472
8		1.912	2.061	96.44	0.77152
Media		1.9115	2.0655	94.14	0.75312
9	10mg Intestini di pesce	1.925	2.196	390.72	3.12576
10		1.928	2.187	382.04	3.05632
Media		1.9265	2.1915	386.38	3.09104

Gel electrophoresis with agarose:

Analisi dei risultati：

The value of A260/A280 was 1.86-1.96, and the value of A260/A230 was above 1.75, which proved that the purity of the extracted product was high; different animal tissue samples had bright bands and good integrity.

Viral Detection “Sharpshooter” – High-Sensitivity Serum/Plasma Kit

Reliably extracts HBV/HCV nucleic acids from 200 μL samples. Delivers leading qPCR CT values for early diagnosis.

Kit per l'estrazione del DNA libero da siero/plasma

Caso sperimentale:

La sostanza standard del siero dell'acido nucleico dell'HCV è stata sottoposta a diluizione con gradiente, e il nostro reagente per l'estrazione dell'acido nucleico del virus del siero/plasma è stato utilizzato per l'estrazione sullo strumento automatico di estrazione e purificazione dell'acido nucleico LJbio32H. I prodotti estratti di varie diluizioni del gradiente di concentrazione sono stati sottoposti al rilevamento qPCR, e i risultati sono i seguenti:

Concentration（IU/ml）	CT		Media	CV
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Analisi dei risultati:

 I dati mostrano un’elevata coerenza, indicando un'eccellente sensibilità e prestazioni stabili del reagente, con un coefficiente di correlazione raggiunto 0.998. L'efficienza di estrazione è notevole, rilevando costantemente sia le concentrazioni basse che quelle alte con stabilità.

“All-inclusive”– The Universal Total RNA Extraction Kit

The universal total RNA extraction reagent is suitable for the rapid and efficient extraction of total RNA from fresh plant, animale, fungal, cellular and other tissue samples. The whole process is convenient to operate, especially suitable for the automated nucleic acid extraction platform.The extracted and purified nucleic acids in this kit can be used for RT-PCR, Real-Time PCR, chip analysis, polyA screening, in vitro translation and molecular cloning.

The universal total RNA extraction reagent

III. Real Data, Real Impact: Client Validation Establishes Benchmark

Caso 1: An agricultural research team used the Plant DNA/RNA Dual Extraction Kit (Cat#231011) on 20 mg sweet potato leaves, raggiungimento 775.2 ng/μL concentration (77.52 μg total yield) E 40% higher PCR efficiency.

Caso 2: CDC testing showed Lingjun’s Serum Virus Kit achieved 100% detection at 10 IU/mL—outperforming competitors (CT values 2–3 cycles earlier).

IV. Limited-Time Empowerment Program: Accelerate Your Research

Act Now – Make Extraction Anxiety-Free!

 Order online: www.lnjnbio.com

 Technical support: 18103799069 / lnjnbio@163.com

 Expo demo: Shanghai International Biotech Expo 2025 (Booth 3-B2108). Live demos + free gel reagents.

Lingjun Commitment: Unlocking nucleic acid challenges with magnetic bead power—making every extraction a trusted data foundation.

Fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 che è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'estrazione e purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza, e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, e così via. Non solo forniamo prodotti, ma possiamo anche intraprendere l'OEM, ODM, e altre esigenze. Se hai un'esigenza correlata, non esitate a contattarci .