Het doorbreken van knelpunten bij de extractie: Lingjun Magnetic Bead RNA-kits herdefiniëren efficiëntienormen Onderzoek mogelijk maken met zeer zuivere nucleïnezuuroplossingen

In moleculair biologisch onderzoek, RNA-extractie uit complexe monsters blijft een cruciaal knelpunt dat de experimentele efficiëntie belemmert. Traditionele methoden worstelen met interferentie van polysacchariden/polyfenolen door planten, risico's op degradatie van dierlijk weefsel, and capturing trace viral nucleic acids—often being time-consuming while compromising purity and yield. With over 13 years of expertise in magnetic bead nucleic acid extraction, Shanghai Lingjun Biotechnology leverages core magnetic bead R&D capabilities to deliver a full-scene magnetic bead RNA extraction kit series, providing researchers with an efficient, safe, and stable one-stop solution.

I. Why Choose Lingjun? Core Technologies Deliver Unmatched Advantages

In-House Magnetic Bead R&D: Industry-Leading Performance

Lingjun’s self-developed superparamagnetic silica beads(1.4μm particle size, 65±5 emu/g magnetic saturation) achieve >95% nucleic acid adsorption through surface modification and functional design. Batch-to-batch consistency eliminates material variability, ensuring reproducible results across laboratories.

“One-Step Protocol” Revolution: Boosts Efficiency by >50%

No centrifugation or toxic reagents: Eliminates hazardous phenol/chloroform and liquid nitrogen grinding. Room-temperature lysis with magnetic bead adsorption delivers pure RNA in under 1 uur.

Full automation compatibility: Kits adapt to mainstream nucleic acid extractors. Magnetic-rod platforms require no manual intervention, inschakelen “laden en gaan” operation.

3.Conquering Four Challenging Samples: Purity Meets Top Journal Standards

Sample Type	Key Innovation	Prestatie (A260/A280)
Polysaccharide/polyphenol plants	Proprietary lysis buffer removes impurities	1.90–2.0 (dandelion leaf)
Animal tissues (muscle/liver)	Grinding-free direct lysis, 100% RNA integrity	1.86–1.96 (shrimp/fish liver)
Serum/plasma viruses	Sensitivity down to 10 IE/ml, repeatability R>0.99	Full detection in gradient dilutions
Trace cells	Extraction from 0.2 µg, zero waste for rare samples	32.7 μg from 20 mg pork

II. Flagship Product Matrix: Precision Solutions for Research Scenarios

Plant RNA “Specialist” – Polysaccharide/Polyphenol Kit

Processes challenging samples (pothos, peach trees, roses) in 30 notulen. Pre-packaged kits (96 tests/box) yield high-purity RNA (A260/A230≥1.8), >9 μg/20 mg leaves—ideal for transcriptome sequencing.

Totale RNA-extractiekit voor planten

Experimenteel geval:

 20mg of sweet potato leaves, peach leaves, rose leaves and eggplant leaves were extracted with LNJNBio plant total RNA extraction kit

No.	Steekproef	A260/A280	A260/A230	Conc.(ng/μL)	Yield（µg）
1	20mg Zoete aardappelbladeren	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Gem		1.974	2.0385	194.1	19.41
3	20mg Peach leaves	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Gem		1.9025	1.4745	159.36	15.936
5	20mg Seasonal leaves	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Gem		1.8565	1.7	99.06	9.906
7	20mg Eggplant leaves	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Gem		1.9875	1.7535	98.34	9.834

Gel electrophoresis with agarose:

Resultaatanalyse： 

The A260/A280 ratio was between 1.8-2.0, while the A260/A230 ratio exceeded 1.4, wat de hoge zuiverheid van de geëxtraheerde producten aangeeft. All plant leaf samples achieved nucleic acid extraction yields above 9 µg. Bright and well-integrated bands were observed in all plant leaf samples. Samengevat, the extraction process yielded high-purity products with substantial output and complete electrophoretic bands when processing different plant leaf samples.

Animal RNA “Guardian” – Degradation-Proof Design

From shrimp to mouse tails, centrifugation-free technology ensures clear 28S/18S bands and 100% electrophoresis integrity. 50-test boxes support high-throughput labs.

Animal Tissue Total RNA Extraction Kit

Experimenteel geval:

The total RNA extraction kit of animal tissues was used to extract shrimp meat, chicken heart, fish heart, fish liver and fish gut respectively

Nee.	Steekproef	A260/A280	A260/A230	Conc.(van/ul)	Yield（μg）
1	20mg garnalen	1.863	1.725	65.48	5.2384
2		1.865	1.782	68.96	5.5168
Gem		1.864	1.7535	67.22	5.3776
3	5mg Chicken heart	1.958	1.721	55.72	0.44576
4		1.966	1.736	56.68	0.45344
Gem		1.962	1.7285	56.2	0.4496
5	5mg Vishart	1.944	1.761	28.56	0.22848
6		1.946	1.742	27.84	0.22272
Gem		1.945	1.7515	28.2	0.2256
7	5mg Vislever	1.911	2.07	91.84	0.73472
8		1.912	2.061	96.44	0.77152
Gem		1.9115	2.0655	94.14	0.75312
9	10mg Fish intestines	1.925	2.196	390.72	3.12576
10		1.928	2.187	382.04	3.05632
Gem		1.9265	2.1915	386.38	3.09104

Gel electrophoresis with agarose:

Resultaatanalyse：

The value of A260/A280 was 1.86-1.96, and the value of A260/A230 was above 1.75, which proved that the purity of the extracted product was high; different animal tissue samples had bright bands and good integrity.

Viral Detection “Sharpshooter” – High-Sensitivity Serum/Plasma Kit

Reliably extracts HBV/HCV nucleic acids from 200 μL samples. Delivers leading qPCR CT values for early diagnosis.

Serum-/plasmavrije DNA-extractiekit

Experimenteel geval:

De standaardsubstantie van HCV-nucleïnezuurserum werd onderworpen aan gradiëntverdunning, en ons serum / plasma-virusnucleïnezuurextractiereagens werd gebruikt voor extractie op het automatische nucleïnezuurextractie- en zuiveringsinstrument LJbio32H. De geëxtraheerde producten van verschillende concentratiegradiëntverdunningen werden onderworpen aan qPCR-detectie, en de resultaten zijn als volgt:

Concentration（IU/ml）	CT		Average	CV
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Resultaatanalyse:

 De gegevens vertonen een hoge consistentie, wat wijst op een uitstekende gevoeligheid en stabiele reagensprestaties, met een correlatiecoëfficiënt die bereikt wordt 0.998. De extractie-efficiëntie is opmerkelijk, consistent detecteren van zowel lage als hoge concentraties met stabiliteit.

“All-inclusive”– The Universal Total RNA Extraction Kit

The universal total RNA extraction reagent is suitable for the rapid and efficient extraction of total RNA from fresh plant, dier, fungal, cellular and other tissue samples. The whole process is convenient to operate, especially suitable for the automated nucleic acid extraction platform.The extracted and purified nucleic acids in this kit can be used for RT-PCR, Real-Time PCR, chip analysis, polyA screening, in vitro translation and molecular cloning.

The universal total RNA extraction reagent

III. Real Data, Real Impact: Client Validation Establishes Benchmark

Geval 1: An agricultural research team used the Plant DNA/RNA Dual Extraction Kit (Cat#231011) on 20 mg sweet potato leaves, achieving 775.2 ng/μL concentration (77.52 μg total yield) En 40% higher PCR efficiency.

Geval 2: CDC testing showed Lingjun’s Serum Virus Kit achieved 100% detection at 10 IU/mL—outperforming competitors (CT values 2–3 cycles earlier).

IV. Limited-Time Empowerment Program: Accelerate Your Research

Act Now – Make Extraction Anxiety-Free!

 Order online: www.lnjnbio.com

 Technical support: 18103799069 / lnjnbio@163.com

 Expo demo: Shanghai International Biotech Expo 2025 (Booth 3-B2108). Live demos + free gel reagents.

Lingjun Commitment: Unlocking nucleic acid challenges with magnetic bead power—making every extraction a trusted data foundation.

Leverancier

Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 dat een professionele fabrikant is van biomagnetische materialen en reagentia voor nucleïnezuurextractie.

We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie, en andere technische gebieden.

Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM ondernemen, ODM, en andere behoeften. Als u een gerelateerde behoefte heeft, Neem gerust contact met ons op .