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Briser les goulots d’étranglement de l’extraction: Les kits d'ARN à billes magnétiques Lingjun redéfinissent les normes d'efficacité en renforçant la recherche grâce à des solutions d'acide nucléique de haute pureté

Dans la recherche en biologie moléculaire, L'extraction d'ARN à partir d'échantillons complexes reste un goulot d'étranglement critique qui entrave l'efficacité expérimentale. Les méthodes traditionnelles luttent contre l’interférence des polysaccharides/polyphénols végétaux, risques de dégradation des tissus animaux, and capturing trace viral nucleic acids—often being time-consuming while compromising purity and yield. With over 13 years of expertise in magnetic bead nucleic acid extraction, Shanghai Lingjun Biotechnology leverages core magnetic bead R&D capabilities to deliver a full-scene magnetic bead RNA extraction kit series, providing researchers with an efficient, sûr, and stable one-stop solution.

je. Why Choose Lingjun? Core Technologies Deliver Unmatched Advantages

In-House Magnetic Bead R&D: Industry-Leading Performance

Lingjun’s self-developed superparamagnetic silica beads(1.4μm particle size, 65±5 emu/g magnetic saturation) achieve >95% nucleic acid adsorption through surface modification and functional design. Batch-to-batch consistency eliminates material variability, ensuring reproducible results across laboratories.

One-Step ProtocolRevolution: Boosts Efficiency by >50%

No centrifugation or toxic reagents: Eliminates hazardous phenol/chloroform and liquid nitrogen grinding. Room-temperature lysis with magnetic bead adsorption delivers pure RNA in under 1 heure.

Full automation compatibility: Kits adapt to mainstream nucleic acid extractors. Magnetic-rod platforms require no manual intervention, enabling “charger et partir” operation.

3.Conquering Four Challenging Samples: Purity Meets Top Journal Standards

Sample TypeKey InnovationPerformance (A260/A280)
Polysaccharide/polyphenol plantsProprietary lysis buffer removes impurities1.90–2.0 (dandelion leaf)
Animal tissues (muscle/liver)Grinding-free direct lysis, 100% RNA integrity1.86–1.96 (shrimp/fish liver)
Serum/plasma virusesSensitivity down to 10 IU/mL, repeatability R>0.99Full detection in gradient dilutions
Trace cellsExtraction from 0.2 µg, zero waste for rare samples32.7 μg from 20 mg pork

II. Flagship Product Matrix: Precision Solutions for Research Scenarios

Plant RNASpecialist– Polysaccharide/Polyphenol Kit

Processes challenging samples (pothos, peach trees, roses) dans 30 minutes. Pre-packaged kits (96 tests/box) yield high-purity RNA (A260/A230≥1.8), >9 μg/20 mg leaves—ideal for transcriptome sequencing.

Kit d’extraction d’ARN total végétal

Experimental case:

 20mg of sweet potato leaves, peach leaves, rose leaves and eggplant leaves were extracted with LNJNBio plant total RNA extraction kit

Non.ÉchantillonA260/A280A260/A230Conc.(ng/μL)Rendement(µg)
120mg Sweet potato leaves1.9722.039193.7619.376
21.9762.038194.4419.444
Moy.1.9742.0385194.119.41
320mg Peach leaves1.9041.477159.1615.916
41.9011.472159.5615.956
Moy.1.90251.4745159.3615.936
520mg Seasonal leaves1.8551.69899.29.92
61.8581.70298.929.892
Moy.1.85651.799.069.906
720mg Eggplant leaves1.9881.75298.089.808
81.9871.75598.69.86
Moy.1.98751.753598.349.834

Gel electrophoresis with agarose:

Analyse des résultats: 

The A260/A280 ratio was between 1.8-2.0, while the A260/A230 ratio exceeded 1.4, indicating high purity of the extracted products. All plant leaf samples achieved nucleic acid extraction yields above 9 µg. Bright and well-integrated bands were observed in all plant leaf samples. En résumé, the extraction process yielded high-purity products with substantial output and complete electrophoretic bands when processing different plant leaf samples.

Animal RNAGuardian– Degradation-Proof Design

From shrimp to mouse tails, centrifugation-free technology ensures clear 28S/18S bands and 100% electrophoresis integrity. 50-test boxes support high-throughput labs.

Animal Tissue Total RNA Extraction Kit

Experimental case:

The total RNA extraction kit of animal tissues was used to extract shrimp meat, chicken heart, fish heart, fish liver and fish gut respectively

Non.ÉchantillonA260/A280A260/A230Conc.(de/ul)Ouichamp(μg)
120mg Crevette1.8631.72565.485.2384
21.8651.78268.965.5168
Moy.1.8641.753567.225.3776
35mg Coeur de poulet1.9581.72155.720.44576
41.9661.73656.680.45344
Moy.1.9621.728556.20.4496
55mg Coeur de poisson1.9441.76128.560.22848
61.9461.74227.840.22272
Moy.1.9451.751528.20.2256
75mg Foie de poisson1.9112.0791.840.73472
81.9122.06196.440.77152
Moy.1.91152.065594.140.75312
910mg Fish intestines1.9252.196390.723.12576
101.9282.187382.043.05632
Moy.1.92652.1915386.383.09104

Gel electrophoresis with agarose:

Analyse des résultats:

The value of A260/A280 was 1.86-1.96, and the value of A260/A230 was above 1.75, which proved that the purity of the extracted product was high; different animal tissue samples had bright bands and good integrity.

Viral DetectionSharpshooter– High-Sensitivity Serum/Plasma Kit

Reliably extracts HBV/HCV nucleic acids from 200 μL samples. Delivers leading qPCR CT values for early diagnosis.

Kit d'extraction d'ADN sans sérum/plasma

Experimental case:

HCV nucleic acid serum standard substance was subjected to gradient dilution, and our serum/plasma virus nucleic acid extraction reagent was used to extract on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of various concentration gradient dilutions were subjected to qPCR detection, et les résultats sont les suivants:

Concentration(IU/ml)CTMoyenne CV
10^723.0222.9622.990.18%
10^625.8825.8325.8550.14%
10^529.0928.9729.030.29%
10^433.1632.4232.791.60%

Analyse des résultats:

 The data shows high consistency, indicating excellent sensitivity and stable reagent performance, with a correlation coefficient reaching 0.998. The extraction efficiency is remarkable, consistently detecting both low and high concentrations with stability.

All-inclusive– The Universal Total RNA Extraction Kit

The universal total RNA extraction reagent is suitable for the rapid and efficient extraction of total RNA from fresh plant, animal, fongique, cellular and other tissue samples. The whole process is convenient to operate, especially suitable for the automated nucleic acid extraction platform.The extracted and purified nucleic acids in this kit can be used for RT-PCR, Real-Time PCR, chip analysis, polyA screening, in vitro translation and molecular cloning.

The universal total RNA extraction reagent

III. Real Data, Real Impact: Client Validation Establishes Benchmark

Cas 1: An agricultural research team used the Plant DNA/RNA Dual Extraction Kit (Cat#231011) on 20 mg sweet potato leaves, atteindre 775.2 ng/μL concentration (77.52 μg total yield) et 40% higher PCR efficiency.

Cas 2: CDC testing showed Lingjun’s Serum Virus Kit achieved 100% detection at 10 IU/mL—outperforming competitors (CT values 2–3 cycles earlier).

IV. Limited-Time Empowerment Program: Accelerate Your Research

Act Now – Make Extraction Anxiety-Free!

 Order online: www.lnjnbio.com

 Technical support: 18103799069 / lnjnbio@163.com

 Expo demo: Shanghai International Biotech Expo 2025 (Booth 3-B2108). Live demos + free gel reagents.

Lingjun Commitment: Unlocking nucleic acid challenges with magnetic bead power—making every extraction a trusted data foundation.

Fournisseur

Société de biotechnologie Shanghai Lingjun., Ltd.a été établi en 2016 qui est un fabricant professionnel de matériaux biomagnétiques et de réactifs d'extraction d'acide nucléique.

Nous avons une riche expérience dans l'extraction et la purification des acides nucléiques, purification des protéines, séparation cellulaire, chimiluminescence, et autres domaines techniques.

Nos produits sont largement utilisés dans de nombreux domaines, comme les tests médicaux, tests génétiques, recherche universitaire, sélection génétique, et ainsi de suite. Nous fournissons non seulement des produits, mais pouvons également entreprendre des OEM, ODM, et autres besoins. Si vous avez un besoin connexe, n'hésitez pas à nous contacter .

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