Comment obtenir une extraction d'ADN de selles à haut rendement avec des billes magnétiques?

Avant-propos

Fecal samples contain a large number of interfering substances, such as bilirubin, bile salts, humic acid and plant polysaccharides, etc.. These impurities can seriously affect the purity and efficiency of DNA extraction.The 160013 silica-based perles magnétiques developed by Shanghai Lingjun Biotechnology Co., Ltée(LnjnBio). provide an efficient and reliable solution to this problem.

1. Core Advantage: Highly efficient adsorption and excellent suspending properties.

Le 160013 silica-based magnetic beads have an average particle size of 1.2µm and are characterised by good suspension (half-settling time up to 20 minutes) and strong magnetic properties (magnetic saturation strength 65±5 emu/g).

Its silica hydroxyl (Si-OH) active group can specifically adsorb nucleic acids, and the recovery rate was up to more than 95% for the target DNA of 20 ng/μL as an example.

2.Working principle: specific binding and release

The product is based on the principle of nucleic acid extraction by magnetic bead method: total DNA is released by lysing human and intestinal flora cells in faeces with high efficiency by digestive solution and protease.

Ensuite, the nano-magnetic beads selectively adsorb the DNA in the lysate under the action of binding solution, and after removing the impurities by washing solution, high quality genomic DNA is released under the action of elution solution.

3. Application features: safe, efficient and flexible compatibility

No need for toxic reagents: the whole process does not require toxic reagents such as phenol and chloroform, and the extracted DNA can be directly used in downstream experiments.

• Forte compatibilité: Suitable for extraction of medium, small or trace samples, especially for application scenarios that require high purity of nucleic acids.
• Flexible operation: can be manually operated or compatible with automated nucleic acid extractors to achieve efficient batch processing.
• Wide sample adaptability: It can handle different volumes of faecal samples ranging from 0.25-5g, and can be adapted to different extraction volumes by adjusting the dosage of the system.

4.Experimental cases:

Total DNA was extracted from 4 different healthy human faecal samples (the upper sample volume was 100 mg for all samples, et 1 sample was made into 2 replicates) en utilisant 160013 silica-based magnetic beads from Shanghai Lingjun Biotechnology Co., Ltée(LnjnBio). on an automatic nucleic acid extraction and purification instrument, LJbio32H.

4.1.Purity and concentration of extracted and purified genomic DNA

No	Échantillon	A260/A280	A260/A230	Conc.. (ng/μL)	Yield（μg）
1	Échantillon 1	1.769	1.041	158.5	15.85
2		1.776	1.109	174.35	17.435
3	Échantillon 2	1.801	1.135	134.35	13.435
4		1.806	1.219	180.95	18.095
56	Échantillon 3	1.784	1.106	166.95	16.695
6		1.797	1.093	133.55	13.355
7	Échantillon 4	1.818	1.136	128.9	12.89
8		1.812	1.123	133.05	13.305

4.2. agarose gel electrophoresis results:

4.3 Experimental Conclusion:

More than 10 μg of total DNA can be captured using LnjnBio 160013 silica-based magnetic beads with good purity and integrity of the extracted target gene.

5 Extension

Based on the above results, Société de biotechnologie Shanghai Lingjun., Ltée(LnjnBio). has launched the fecal total DNA extraction reagent (méthode des billes magnétiques), which can quickly and efficiently extract the total DNA of intestinal flora from fecal samples, and the extracted DNA can be directly used in downstream experiments, such as PCR, sequencing and so on.

En outre, LnjnBio 160013 in silico magnetic beads are not only suitable for faecal genomic DNA extraction, but also can be used for whole blood genomic DNA extraction and purification, viral DNA/RNA isolation and purification, small fragment DNA/RNA isolation and various trace nucleic acid isolation and purification.

Fournisseur

Société de biotechnologie Shanghai Lingjun., Ltd.a été établi en 2016 qui est un fabricant professionnel de matériaux biomagnétiques et de réactifs d'extraction d'acide nucléique.

Nous avons une riche expérience dans l'extraction et la purification des acides nucléiques, purification des protéines, séparation cellulaire, chimiluminescence, et autres domaines techniques.

Nos produits sont largement utilisés dans de nombreux domaines, comme les tests médicaux, tests génétiques, recherche universitaire, sélection génétique, et ainsi de suite. Nous fournissons non seulement des produits, mais pouvons également entreprendre des OEM, ODM, et autres besoins. Si vous avez un besoin connexe, n'hésitez pas à nous contacter .