Romper los cuellos de botella en la extracción: Los kits de ARN de perlas magnéticas de Lingjun redefinen los estándares de eficiencia Potenciando la investigación con soluciones de ácido nucleico de alta pureza

En la investigación de biología molecular., La extracción de ARN de muestras complejas sigue siendo un cuello de botella crítico que obstaculiza la eficiencia experimental. Los métodos tradicionales luchan contra la interferencia de polisacáridos y polifenoles de las plantas, riesgos de degradación del tejido animal, and capturing trace viral nucleic acids—often being time-consuming while compromising purity and yield. With over 13 years of expertise in magnetic bead nucleic acid extraction, Shanghai Lingjun Biotechnology leverages core magnetic bead R&D capabilities to deliver a full-scene magnetic bead RNA extraction kit series, providing researchers with an efficient, safe, and stable one-stop solution.

I. Why Choose Lingjun? Core Technologies Deliver Unmatched Advantages

In-House Magnetic Bead R&D: Industry-Leading Performance

Lingjun’s self-developed superparamagnetic silica beads(1.4μm particle size, 65±5 emu/g magnetic saturation) achieve >95% nucleic acid adsorption through surface modification and functional design. Batch-to-batch consistency eliminates material variability, ensuring reproducible results across laboratories.

“One-Step Protocol” Revolution: Boosts Efficiency by >50%

No centrifugation or toxic reagents: Eliminates hazardous phenol/chloroform and liquid nitrogen grinding. Room-temperature lysis with magnetic bead adsorption delivers pure RNA in under 1 hora.

Full automation compatibility: Kits adapt to mainstream nucleic acid extractors. Magnetic-rod platforms require no manual intervention, habilitando “cargar y listo” operation.

3.Conquering Four Challenging Samples: Purity Meets Top Journal Standards

Sample Type	Key Innovation	Performance (A260/A280)
Polysaccharide/polyphenol plants	Proprietary lysis buffer removes impurities	1.90–2.0 (dandelion leaf)
Animal tissues (muscle/liver)	Grinding-free direct lysis, 100% RNA integrity	1.86–1.96 (shrimp/fish liver)
Serum/plasma viruses	Sensitivity down to 10 IU/mL, repeatability R>0.99	Full detection in gradient dilutions
Trace cells	Extraction from 0.2 µg, zero waste for rare samples	32.7 μg from 20 mg pork

II. Flagship Product Matrix: Precision Solutions for Research Scenarios

Plant RNA “Specialist” – Polysaccharide/Polyphenol Kit

Processes challenging samples (pothos, peach trees, roses) en 30 minutos. Pre-packaged kits (96 tests/box) yield high-purity RNA (A260/A230≥1.8), >9 μg/20 mg leaves—ideal for transcriptome sequencing.

Kit de extracción de ARN total de plantas

Caso experimental:

 20mg of sweet potato leaves, peach leaves, rose leaves and eggplant leaves were extracted with LNJNBio plant total RNA extraction kit

No.	Muestra	A260/A280	A260/A230	Conc.(ng/μL)	Rendimiento（μg）
1	20mg Sweet potato leaves	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
promedio		1.974	2.0385	194.1	19.41
3	20mg Peach leaves	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
promedio		1.9025	1.4745	159.36	15.936
5	20mg Seasonal leaves	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
promedio		1.8565	1.7	99.06	9.906
7	20mg Eggplant leaves	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
promedio		1.9875	1.7535	98.34	9.834

Gel electrophoresis with agarose:

Análisis de resultados： 

The A260/A280 ratio was between 1.8-2.0, while the A260/A230 ratio exceeded 1.4, indicating high purity of the extracted products. All plant leaf samples achieved nucleic acid extraction yields above 9 µg. Bright and well-integrated bands were observed in all plant leaf samples. En resumen, the extraction process yielded high-purity products with substantial output and complete electrophoretic bands when processing different plant leaf samples.

Animal RNA “Guardian” – Degradation-Proof Design

From shrimp to mouse tails, centrifugation-free technology ensures clear 28S/18S bands and 100% electrophoresis integrity. 50-test boxes support high-throughput labs.

Kit de extracción de ARN total de tejido animal

Caso experimental:

The total RNA extraction kit of animal tissues was used to extract shrimp meat, chicken heart, fish heart, fish liver and fish gut respectively

No.	Muestra	A260/A280	A260/A230	Conc.(de/ul)	Yield（μg）
1	20mg de camarones	1.863	1.725	65.48	5.2384
2		1.865	1.782	68.96	5.5168
promedio		1.864	1.7535	67.22	5.3776
3	5mg de corazón de pollo	1.958	1.721	55.72	0.44576
4		1.966	1.736	56.68	0.45344
promedio		1.962	1.7285	56.2	0.4496
5	5mg corazón de pescado	1.944	1.761	28.56	0.22848
6		1.946	1.742	27.84	0.22272
promedio		1.945	1.7515	28.2	0.2256
7	5mg de hígado de pescado	1.911	2.07	91.84	0.73472
8		1.912	2.061	96.44	0.77152
promedio		1.9115	2.0655	94.14	0.75312
9	10mg de intestinos de pescado	1.925	2.196	390.72	3.12576
10		1.928	2.187	382.04	3.05632
promedio		1.9265	2.1915	386.38	3.09104

Gel electrophoresis with agarose:

Análisis de resultados：

The value of A260/A280 was 1.86-1.96, and the value of A260/A230 was above 1.75, which proved that the purity of the extracted product was high; different animal tissue samples had bright bands and good integrity.

Viral Detection “Sharpshooter” – High-Sensitivity Serum/Plasma Kit

Reliably extracts HBV/HCV nucleic acids from 200 μL samples. Delivers leading qPCR CT values for early diagnosis.

Kit de extracción de ADN sin suero/plasma

Caso experimental:

HCV nucleic acid serum standard substance was subjected to gradient dilution, and our serum/plasma virus nucleic acid extraction reagent was used to extract on the automatic nucleic acid extraction and purification instrument LJbio32H. The extracted products of various concentration gradient dilutions were subjected to qPCR detection, y los resultados son los siguientes:

dooncentration（IU/ml）	CT		Promedio	CV
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Análisis de resultados:

 The data shows high consistency, indicating excellent sensitivity and stable reagent performance, with a correlation coefficient reaching 0.998. The extraction efficiency is remarkable, consistently detecting both low and high concentrations with stability.

“All-inclusive”– The Universal Total RNA Extraction Kit

The universal total RNA extraction reagent is suitable for the rapid and efficient extraction of total RNA from fresh plant, animal, hongos, cellular and other tissue samples. The whole process is convenient to operate, especially suitable for the automated nucleic acid extraction platform.The extracted and purified nucleic acids in this kit can be used for RT-PCR, Real-Time PCR, chip analysis, polyA screening, in vitro translation and molecular cloning.

The universal total RNA extraction reagent

III. Real Data, Real Impact: Client Validation Establishes Benchmark

Caso 1: An agricultural research team used the Plant DNA/RNA Dual Extraction Kit (Cat#231011) on 20 mg sweet potato leaves, logrando 775.2 ng/μL concentration (77.52 μg total yield) y 40% higher PCR efficiency.

Caso 2: CDC testing showed Lingjun’s Serum Virus Kit achieved 100% detection at 10 IU/mL—outperforming competitors (CT values 2–3 cycles earlier).

IV. Limited-Time Empowerment Program: Accelerate Your Research

Act Now – Make Extraction Anxiety-Free!

 Order online: www.lnjnbio.com

 Technical support: 18103799069 / lnjnbio@163.com

 Expo demo: Shanghai International Biotech Expo 2025 (Booth 3-B2108). Live demos + free gel reagents.

Lingjun Commitment: Unlocking nucleic acid challenges with magnetic bead power—making every extraction a trusted data foundation.

Proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 que es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes una necesidad relacionada, no dude en contactarnos .