Quebrando gargalos de extração: Os kits de RNA de esferas magnéticas Lingjun redefinem os padrões de eficiência, capacitando a pesquisa com soluções de ácido nucleico de alta pureza

Na pesquisa de biologia molecular, A extração de RNA de amostras complexas continua sendo um gargalo crítico que dificulta a eficiência experimental. Métodos tradicionais lutam contra a interferência de polissacarídeos/polifenóis vegetais, riscos de degradação de tecidos animais, and capturing trace viral nucleic acids—often being time-consuming while compromising purity and yield. With over 13 years of expertise in magnetic bead nucleic acid extraction, Shanghai Lingjun Biotechnology leverages core magnetic bead R&D capabilities to deliver a full-scene magnetic bead RNA extraction kit series, providing researchers with an efficient, safe, and stable one-stop solution.

EU. Why Choose Lingjun? Core Technologies Deliver Unmatched Advantages

In-House Magnetic Bead R&D: Industry-Leading Performance

Lingjun’s self-developed superparamagnetic silica beads(1.4μm particle size, 65±5 emu/g magnetic saturation) achieve >95% nucleic acid adsorption through surface modification and functional design. Batch-to-batch consistency eliminates material variability, ensuring reproducible results across laboratories.

“One-Step Protocol” Revolution: Boosts Efficiency by >50%

No centrifugation or toxic reagents: Eliminates hazardous phenol/chloroform and liquid nitrogen grinding. Room-temperature lysis with magnetic bead adsorption delivers pure RNA in under 1 hora.

Full automation compatibility: Kits adapt to mainstream nucleic acid extractors. Magnetic-rod platforms require no manual intervention, habilitando “carregar e ir” operation.

3.Conquering Four Challenging Samples: Purity Meets Top Journal Standards

Sample Type	Key Innovation	Desempenho (A260/A280)
Polysaccharide/polyphenol plants	Proprietary lysis buffer removes impurities	1.90–2.0 (dandelion leaf)
Animal tissues (muscle/liver)	Grinding-free direct lysis, 100% RNA integrity	1.86–1.96 (shrimp/fish liver)
Serum/plasma viruses	Sensitivity down to 10 UI/mL, repeatability R>0.99	Full detection in gradient dilutions
Trace cells	Extraction from 0.2 μg, zero waste for rare samples	32.7 μg from 20 mg pork

II. Flagship Product Matrix: Precision Solutions for Research Scenarios

Plant RNA “Specialist” – Polysaccharide/Polyphenol Kit

Processes challenging samples (pothos, peach trees, roses) em 30 minutos. Pre-packaged kits (96 tests/box) yield high-purity RNA (A260/A230≥1.8), >9 μg/20 mg leaves—ideal for transcriptome sequencing.

Kit de extração de RNA total de planta

Caso experimental:

 20mg of sweet potato leaves, peach leaves, rose leaves and eggplant leaves were extracted with LNJNBio plant total RNA extraction kit

Não.	Amostra	A260/A280	A260/A230	Conc.(ng/µL)	Rendimento（µg）
1	20mg Folhas de batata doce	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Média		1.974	2.0385	194.1	19.41
3	20mg Folhas de pêssego	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Média		1.9025	1.4745	159.36	15.936
5	20mg Folhas sazonais	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Média		1.8565	1.7	99.06	9.906
7	20mg Folhas de berinjela	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Média		1.9875	1.7535	98.34	9.834

Gel electrophoresis with agarose:

Análise de resultados： 

The A260/A280 ratio was between 1.8-2.0, while the A260/A230 ratio exceeded 1.4, indicando alta pureza dos produtos extraídos. All plant leaf samples achieved nucleic acid extraction yields above 9 μg. Bright and well-integrated bands were observed in all plant leaf samples. Resumindo, the extraction process yielded high-purity products with substantial output and complete electrophoretic bands when processing different plant leaf samples.

Animal RNA “Guardian” – Degradation-Proof Design

From shrimp to mouse tails, centrifugation-free technology ensures clear 28S/18S bands and 100% electrophoresis integrity. 50-test boxes support high-throughput labs.

Animal Tissue Total RNA Extraction Kit

Caso experimental:

The total RNA extraction kit of animal tissues was used to extract shrimp meat, chicken heart, fish heart, fish liver and fish gut respectively

Não.	Amostra	A260/A280	A260/A230	Conc.(de/ul)	Sield（μg）
1	20mg Shrimp	1.863	1.725	65.48	5.2384
2		1.865	1.782	68.96	5.5168
Média		1.864	1.7535	67.22	5.3776
3	5mg Coração de frango	1.958	1.721	55.72	0.44576
4		1.966	1.736	56.68	0.45344
Média		1.962	1.7285	56.2	0.4496
5	5mg Fish heart	1.944	1.761	28.56	0.22848
6		1.946	1.742	27.84	0.22272
Média		1.945	1.7515	28.2	0.2256
7	5mg Fígado de peixe	1.911	2.07	91.84	0.73472
8		1.912	2.061	96.44	0.77152
Média		1.9115	2.0655	94.14	0.75312
9	10mg Fish intestines	1.925	2.196	390.72	3.12576
10		1.928	2.187	382.04	3.05632
Média		1.9265	2.1915	386.38	3.09104

Gel electrophoresis with agarose:

Análise de resultados：

The value of A260/A280 was 1.86-1.96, and the value of A260/A230 was above 1.75, which proved that the purity of the extracted product was high; different animal tissue samples had bright bands and good integrity.

Viral Detection “Sharpshooter” – High-Sensitivity Serum/Plasma Kit

Reliably extracts HBV/HCV nucleic acids from 200 μL samples. Delivers leading qPCR CT values for early diagnosis.

Kit de extração de DNA sem soro/plasma

Caso experimental:

A substância padrão sérica de ácido nucleico do HCV foi submetida a diluição gradiente, e nosso reagente de extração de ácido nucleico de soro/plasma vírus foi usado para extrair no instrumento automático de extração e purificação de ácido nucleico LJbio32H. Os produtos extraídos de várias diluições de gradiente de concentração foram submetidos à detecção de qPCR, e os resultados são os seguintes:

Concentration（IU/ml）	CT		Média	CV
10^7	23.02	22.96	22.99	0.18%
10^6	25.88	25.83	25.855	0.14%
10^5	29.09	28.97	29.03	0.29%
10^4	33.16	32.42	32.79	1.60%

Análise de resultados:

 Os dados mostram alta consistência, indicando excelente sensibilidade e desempenho estável do reagente, com um coeficiente de correlação atingindo 0.998. A eficiência de extração é notável, detectando consistentemente concentrações baixas e altas com estabilidade.

“All-inclusive”– The Universal Total RNA Extraction Kit

The universal total RNA extraction reagent is suitable for the rapid and efficient extraction of total RNA from fresh plant, animal, fúngico, cellular and other tissue samples. The whole process is convenient to operate, especially suitable for the automated nucleic acid extraction platform.The extracted and purified nucleic acids in this kit can be used for RT-PCR, PCR em tempo real, chip analysis, triagem poliA, tradução in vitro e clonagem molecular.

The universal total RNA extraction reagent

III. Real Data, Real Impact: Client Validation Establishes Benchmark

Caso 1: An agricultural research team used the Plant DNA/RNA Dual Extraction Kit (Cat#231011) on 20 mg sweet potato leaves, alcançar 775.2 ng/μL concentration (77.52 μg total yield) e 40% higher PCR efficiency.

Caso 2: CDC testing showed Lingjun’s Serum Virus Kit achieved 100% detection at 10 IU/mL—outperforming competitors (CT values 2–3 cycles earlier).

4. Limited-Time Empowerment Program: Accelerate Your Research

Act Now – Make Extraction Anxiety-Free!

 Order online: www.lnjnbio.com

 Technical support: 18103799069 / lnjnbio@163.com

 Expo demo: Shanghai International Biotech Expo 2025 (Booth 3-B2108). Live demos + free gel reagents.

Lingjun Commitment: Unlocking nucleic acid challenges with magnetic bead power—making every extraction a trusted data foundation.

Fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 que é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência, e outras áreas técnicas.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver uma necessidade relacionada, não hesite em contactar-nos .