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Van Hot Spring tot DNA-identificatie

In the summer of 1966, Hudson Fritz, an undergraduate at Indiana University, moved into a cabin on the edge of Yellowstone National Park. He was then working for microbiologist Thomas Brock.

Brooke was convinced that certain microorganisms could survive in temperatures far exceeding expectations. Consequently, Fritz made daily trips to the hot spring area to collect bacterial samples. Op september 19 of that year, he successfully cultured a pale yellow microbial sample from the Mushroom Hot Spring. Under the microscope, he observed abundant cells collected from water near boiling point.

Figuur 1: A corner of Yellowstone National Park in the United States

I saw something my predecessors had never seen,” recalls Fritz, now at the Sandford Burnham Prebises Institute for Medical Discovery in California. “To this day, the sight of that microscope image still sends shivers down my spine.

Three years later, Fritz and Brock described one of these bacteria, naming itThermophilus aquaticus.This bacterium thrives optimally at 70°C. In 1976, a team from the University of Cincinnati, Ohio, isolated an enzyme from Thermophilus aquaticus—aDNA polymerasecapable of synthesizing new deoxyribonucleic acid (DNA) at 80°C.

Seven years later, a substance dubbedTaq polymeraseproved ideal for biochemist Kary Mullis to develop the polymerase chain reaction (PCR) techniek, which can rapidly amplify a single DNA fragment into thousands of copies. Since Mullis required high temperatures to denature DNA strands, he needed a polymerase capable of functioning at elevated temperatures.

Vandaag, PCR technology has become an indispensable tool, widely applied across multiple fields. In the medical field, it is used for organ transplant matching and cancer diagnosis; in criminal investigation, it enables DNA fingerprint identification. De PCR amplification reagents developed by Shanghai Lingjun Biotechnology are also extensively used in teaching experiments, earning high praise from both teachers and students.

Shanghai Lingjun Bio PCR-amplificatiekit

Leverancier

Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 dat een professionele fabrikant is van biomagnetische materialen en reagentia voor nucleïnezuurextractie.

We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie, en andere technische gebieden.

Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM ondernemen, ODM, en andere behoeften. Als u een gerelateerde behoefte heeft, Neem gerust contact met ons op .

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