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Fra Hot Spring til DNA-identifikasjon

Om sommeren 1966, Hudson Fritz, en undergraduate ved Indiana University, flyttet inn i en hytte i utkanten av Yellowstone nasjonalpark. Han jobbet da for mikrobiolog Thomas Brock.

Brooke was convinced that certain microorganisms could survive in temperatures far exceeding expectations. Følgelig, Fritz made daily trips to the hot spring area to collect bacterial samples. På september 19 of that year, he successfully cultured a pale yellow microbial sample from the Mushroom Hot Spring. Under the microscope, he observed abundant cells collected from water near boiling point.

Figur 1: A corner of Yellowstone National Park in the United States

I saw something my predecessors had never seen,” recalls Fritz, now at the Sandford Burnham Prebises Institute for Medical Discovery in California. “To this day, the sight of that microscope image still sends shivers down my spine.

Three years later, Fritz and Brock described one of these bacteria, naming itThermophilus aquaticus.This bacterium thrives optimally at 70°C. I 1976, a team from the University of Cincinnati, Ohio, isolated an enzyme from Thermophilus aquaticus—aDNA polymerasecapable of synthesizing new deoxyribonucleic acid (DNA) at 80°C.

Seven years later, a substance dubbed “Taq polymerase” proved ideal for biochemist Kary Mullis to develop the polymerase chain reaction (PCR) teknikk, which can rapidly amplify a single DNA fragment into thousands of copies. Since Mullis required high temperatures to denature DNA strands, he needed a polymerase capable of functioning at elevated temperatures.

I dag, PCR technology has become an indispensable tool, widely applied across multiple fields. På det medisinske feltet, it is used for organ transplant matching and cancer diagnosis; in criminal investigation, it enables DNA fingerprint identification. De PCR amplification reagents developed by Shanghai Lingjun Biotechnology are also extensively used in teaching experiments, earning high praise from both teachers and students.

Shanghai Lingjun Bio PCR Amplification Kit

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.ble etablert i 2016 som er en profesjonell produsent av biomagnetiske materialer og nukleinsyreekstraksjonsreagenser.

Vi har rik erfaring innen utvinning og rensing av nukleinsyre, proteinrensing, celleseparasjon, kjemiluminescens, og andre tekniske felt.

Våre produkter er mye brukt på mange felt, som medisinsk testing, genetisk testing, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke bare produkter, men kan også påta oss OEM, ODM, og andre behov. Hvis du har et relatert behov, ta gjerne kontakt med oss .

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