Teknologi pengekstrakan asid nukleik berasaskan manik magnet: Leading the efficiency revolution in the biopharmaceutical industry

In the current era of surging demands for precision medicine and large-scale disease screening, a key technology is quietly driving the efficiency revolution in the biopharmaceutical industry – pengekstrakan asid nukleik berasaskan manik magnet. With its significant advantages of high efficiency, automasi, and high purity, this technology has rapidly become an indispensable core tool in molecular diagnostics, penjujukan gen, and new drug development fields.

Menggunakan reagen pengekstrakan asid nukleik virus serum/plasma kami, Pencairan kecerunan piawai kebangsaan HBV telah diekstrak pada pengekstrakan asid nukleik automatik dan instrumen penulenan LJbio32H. Produk yang diekstrak bagi setiap pencair kecerunan kepekatan telah dikesan oleh HBV-qPCR, dengan pekali korelasi R>0.99. Data khusus adalah seperti berikut:

Analisis keputusan：Ketepatan reagen adalah sangat tinggi, dan sensitiviti masih baik apabila mengekstrak sampel berkepekatan rendah.

Compared with the traditional centrifugal column method, magnetic bead-based extraction achieves rapid separation and purification through the specific binding of nucleic acids to surface-modified magnetic particles under the action of an external magnetic field. This principle enables breakthroughs in throughput, kelajuan, and consistency. Dalam beberapa tahun kebelakangan ini, this technology has excelled in scenarios such as large-scale screening of infectious diseases (such as COVID-19 nucleic acid testing), tumor liquid biopsy (microscopic ctDNA capture), and genetic disease analysis, effectively supporting high-throughput, standardized modern testing requirements.

Asid nukleik diekstrak daripada jumlah surih sel Hela manusia sebagai sampel, 5 sel, 10 sel, 20 sel, 500 sel telah diekstrak, dan asid nukleik yang diperolehi dianalisis menggunakan Agilent 4200 Taburan serpihan asid nukleik, dan keputusan yang diperoleh ditunjukkan di bawah: 

1. Elektroferogram Agilent 4200 Ujian Pita Skrin RNA Sensitiviti Tinggi

D1: 50 sel helaG1: 20 sel helaB2: 10 sel helaE2: 5 sel hela

2. Gambar rajah puncak bagi 50/20/10/5 Sel Hela diekstrak

• Gambar rajah puncak bagi 5 Sel Hela diekstrak

• . keputusan ujian Qubit

Tidak.	Pengambilan sampel	hasil(daripada/μL）
1	50 sel hela	0.242
2	20 sel hela	0.136
3	10 sel hela	0.112
4	5 sel hela	0.096

Analisis keputusan： Oleh Agilent 4200 keputusan elektroforesis dapat dilihat: 5– 50 kesan sampel sel Hela manusia, boleh diperolehi oleh serpihan asid nukleik, Produk pengekstrakan ialah RNA 18s, 28sRNA, dan DNA melebihi 800bp.

The wave of industry innovation continues to surge. Domestic leading enterprises have launched fully automated solutions integrating magnetic bead extraction, mencapai “sample input and result output” in closed tubes, reducing extraction time to minutes and significantly enhancing emergency testing capabilities. Pada masa yang sama, breakthroughs in domestic high-end magnetic bead raw materials, such as those from companies like Shanghai Lingjun Biotechnology, have broken the import monopoly, reducing the cost of a single sample by over 30%, significantly enhancing the autonomy of the industrial chain. Capital markets have shown great favor for this sector, with many related enterprises completing large-scale financing recently, driving technological iteration into a fast lane.

Melihat ke masa hadapan, with the explosion of personalized medical care and immediate testing (POCT) demands, magnetic bead-based technology is evolving towards higher sensitivity, better anti-interference performance, and green biodegradability. Its combination with microfluidics and artificial intelligence is expected to give rise to a new generation of intelligent extraction platforms, continuously providing key infrastructure for the innovation and development of the biopharmaceutical industry.

Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 yang merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence, dan bidang teknikal yang lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami .