একটি সাধারণ-উদ্দেশ্য চৌম্বক পুঁতি-ভিত্তিক সর্বজনীন মোট আরএনএ নিষ্কাশন কিটের ব্যবহারিক মূল্যায়ন

As we know, আরএনএ নিষ্কাশন is a basic procedure, the results are often fraught with uncertainty. This is particularly true of animal tissue samples: different tissues—such as liver, muscle, brain and spleen—vary significantly in texture, endogenous enzyme content and fat content, and the slightest misstep can lead to RNA degradation or insufficient purity. Many laboratories are forced to stock multiple kits for different tissues, which not only takes up space but also leads to confusion; should the extraction of a precious sample fail, it can seriously disrupt the progress of the experiment.

আজ, we carried out RNA extraction from animal tissue using the newly launched magnetic bead-Based universal total RNA extraction kit from Shanghai Lingjun Biotechnology. The instrument used was the LJbio32H automated nucleic acid extraction and purification system. পরীক্ষামূলক ফলাফল নিম্নরূপ:

Agarose gel electrophoresis（1.5% 120V 25min）：

বিশুদ্ধতার দিক দিয়ে, yield and the integrity of the RNA bands, this kit delivers excellent extraction results, making it fully capable of meeting customers’ requirements for demanding downstream experiments such as cDNA library construction, qPCR, Northern blotting and RNA-seq.

The core of this kit lies in its unique lysis system and magnetic bead purification technology. The entire process requires no phenol/chloroform extraction; instead, it relies on an optimised lysis buffer to rapidly disrupt a variety of animal tissues—including liver, muscle, brain, heart and spleen—thereby fully releasing RNA whilst effectively inhibiting RNase activity. Magnetic beads specifically designed to bind nucleic acids are then added; নির্দিষ্ট বাফার অবস্থার অধীনে, the RNA binds efficiently to the surface of the magnetic beads. Solid-liquid separation is easily achieved by applying a magnetic field via a magnetic stand. After a simple washing step to remove impurities such as proteins and lipids, the RNA is eluted using enzyme-free water to yield high-purity total RNA. The entire manual procedure is straightforward and takes approximately 25–30 minutes. The kit’s protocol can also be easily adapted for use with various automated nucleic acid extraction systems, enabling standardised extraction at higher throughput.

The product incorporates a highly efficient genomic DNA removal step, which significantly reduces gDNA residue, ensuring clearer and more reliable negative control results in downstream RT-qPCR. The instruction manual also thoughtfully provides recommended tissue quantities and lysis times tailored to the characteristics of different animal tissues, enabling users to get started smoothly even when processing fibrous muscle tissue or fatty brain tissue for the first time.

In practical terms, this universal magnetic bead-based RNA extraction kit from Shanghai Lingjun is suitable for a wide range of animal tissue samples, including liver, muscle, brain, heart, প্লীহা, lung and kidney, truly offering the convenience of requiring just one flagship kit per laboratory.

At a time when reagent supply is often subject to fluctuations, Shanghai Lingjun Biotechnology, as a leading domestic supplier of high-quality nucleic acid extraction reagents, offers this magnetic bead-based universal RNA extraction kit, which not only delivers outstanding performance but also boasts impressive delivery speed and value for money. উপরন্তু, a dedicated technical support team is on hand to resolve any issues that may arise during the extraction process.

সরবরাহকারী

সাংহাই লিংজুন বায়োটেকনোলজি কো., লিমিটেডসালে প্রতিষ্ঠিত হয় 2016 যা বায়োম্যাগনেটিক উপকরণ এবং নিউক্লিক অ্যাসিড নিষ্কাশন বিকারকগুলির একটি পেশাদার প্রস্তুতকারক.

আমাদের নিউক্লিক অ্যাসিড নিষ্কাশন এবং পরিশোধন সমৃদ্ধ অভিজ্ঞতা আছে, প্রোটিন পরিশোধন, কোষ বিচ্ছেদ, কেমিলুমিনেসেন্স, এবং অন্যান্য প্রযুক্তিগত ক্ষেত্র.

আমাদের পণ্য ব্যাপকভাবে অনেক ক্ষেত্রে ব্যবহৃত হয়, যেমন মেডিকেল টেস্টিং, জেনেটিক পরীক্ষা, বিশ্ববিদ্যালয় গবেষণা, জেনেটিক প্রজনন, এবং তাই. আমরা কেবল পণ্য সরবরাহ করি না তবে OEM গ্রহণ করতে পারি, ওডিএম, এবং অন্যান্য প্রয়োজন. আপনি যদি একটি সম্পর্কিত প্রয়োজন আছে, আমাদের সাথে যোগাযোগ বিনা দ্বিধায় দয়া করে .