Introduction to total RNA extraction reagent

Total RNA extraction reagent is a scientific research tool designed for efficient and comprehensive separation of all RNA molecules from various biological samples. It combines advanced biochemical technology and optimized extraction process to ensure the high purity and integrity of various RNA molecules such as mRNA, rRNA, miRNA, etc., and provides a solid foundation for in-depth exploration in the fields of transcriptomics research, gene expression analysis, disease diagnosis, etc.

Features and advantages of total RNA extraction reagent

Comprehensive coverage: It can extract all types of RNA including mRNA, rRNA, miRNA from samples to meet diverse research needs.

High purity guarantee: It adopts a unique decontamination formula to effectively remove DNA residues and protein impurities to ensure the high purity of RNA, which is suitable for downstream high-sensitivity experiments.

Strong stability: Optimized storage conditions and reagent stability can maintain high efficiency even under long-term storage and reduce batch-to-batch differences.

Convenient operation: Standardized operation procedures greatly simplify the experimental steps, and can maintain consistent extraction efficiency even in high-throughput processing scenarios.

Wide applicability: Whether it is animal cells, plant tissues, microbial samples, or clinical blood and tissue specimens, excellent RNA recovery rates can be achieved.

Properties of total RNA extraction reagents

Total RNA extraction reagents are mainly composed of lysis solution, RNA protective agent, phase separation solution, washing solution and dissolving solution. The lysis solution and RNA protective agent work together to quickly destroy the cell structure, while inhibiting the activity of endogenous RNases and protecting RNA from degradation; the phase separation solution promotes the separation of RNA and cell residues for subsequent purification; the washing solution removes residual impurities and improves the purity of RNA; the dissolving solution is used for the final dissolution of RNA to ensure that it is in a suitable experimental state.

Application of total RNA extraction reagents

Transcriptomics research: whole transcriptome sequencing, differentially expressed gene analysis, non-coding RNA research.

Clinical diagnosis: tumor marker detection, viral infection monitoring, genetic disease gene expression analysis.

Plant and microbiology: gene regulatory network analysis, environmental response mechanism exploration, metabolic pathway research.

Pharmacology and toxicology: exploration of drug action mechanisms, gene expression changes in toxicity assessment.

Biotechnology industry: bioengineering transformation, safety evaluation of genetically modified crops.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us sales1@lingjunbio.com.

FAQs of total RNA extraction reagents

Q: Is the total RNA extraction reagent suitable for trace samples?

A: Yes, many total RNA extraction reagents have optimized small-volume operation processes, and can achieve high-efficiency RNA recovery even for trace samples.

Q: Can the extracted total RNA be used directly for RT-qPCR?

A: After quality assessment (such as A260/A280 ratio detection) and confirmation of no DNA contamination, the extracted total RNA can be directly used for downstream experiments such as RT-qPCR.

Q: How to evaluate the purity and integrity of total RNA?

A: Purity is usually assessed by measuring the A260/A280 ratio with a UV spectrophotometer, and RNA integrity is detected by gel electrophoresis or Bioanalyzer.

Q: How to prevent RNA degradation during total RNA extraction?

A: It must be operated under RNase-free conditions, use RNase-free consumables and workbenches, keep the samples low temperature throughout the process, and add RNase inhibitors in time.

Q: How to store the extracted total RNA for a long time?

A: High-purity total RNA should be stored at -70℃ or lower to avoid repeated freezing and thawing to maintain the stability and integrity of RNA.

Total RNA extraction reagents are an indispensable tool for modern molecular biology research. Their performance is directly related to the quality and reliability of scientific research results. We are committed to providing the most cutting-edge and reliable total RNA extraction solutions to help you on your scientific exploration journey. If you need to learn more about product details or seek technical guidance, please feel free to contact us.