Fordele ved alkoholfrit nukleinsyreekstraktionsreagens

1. Main advantages of alcohol-free extraction reagents

To avoid the risks associated with organic solvents, traditionelle ekstraktionsreagenser er afhængige af ethanol eller isopropanol som nukleinsyrestabilisatorer. Imidlertid, disse alkoholer er brandfarlige, eksplosiv, and volatile, requiring special conditions for transportation and storage (such as low temperatures and explosion-proof packaging). I modsætning hertil, alcohol-free reagents eliminate these safety hazards through alternative formulations(such as polyethylene glycol and oil phases), reducing transportation costs and enhancing the stability of the reagent after opening.

Improving operational convenience and compatibility with automation, alcohol-containing reagents require manual intervention for addition in automated processes, whereas alcohol-free reagents can be preloaded directly into the kit, enabling fully enclosed automated operations. F.eks, a certain blood nucleic acid extraction kit optimizes the lysis and rinse solution formulations to complete the magnetic bead-bound nucleic acid step without pausing the instrument, reducing the extraction time to within 20 minutter.

Reduktion af resterende interferens, ethanol residues can inhibit downstream PCR and other enzymatic reactions, whereas alcohol-free reagents effectively remove impurities through washing liquid stratification techniques (such as the synergy between aqueous and oily phases), avoiding the negative impact of residues on detection sensitivity. F.eks, a patented technology achieves efficient nucleic acid purification by washing with PEG in one step, increasing the recovery rate by four times compared to traditional methods.

Simplifying the formula and reducing costs, traditional alcohol-free solutions often require complex components (such as proteinase K). I modsætning hertil, new reagents reduce the number of reagents and lower production costs by optimizing PEG concentration and sodium salt ratios. F.eks, a magnetic bead kit only requires lysis buffer, wash buffer A/B, and elution buffer, eliminating the need for additional proteinase K, significantly reducing the complexity of the kit.

To enhance environmental friendliness, alcohol-free reagents avoid the use of toxic organic solvents, in line with the trend of green chemistry.

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens, og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har et relateret behov, er du velkommen til at kontakte os .