Keuntungan dari reagen ekstraksi asam nukleat bebas alkohol

1. Main advantages of alcohol-free extraction reagents

To avoid the risks associated with organic solvents, reagen ekstraksi tradisional mengandalkan etanol atau isopropanol sebagai penstabil asam nukleat. Namun, alkohol ini mudah terbakar, eksplosif, and volatile, requiring special conditions for transportation and storage (such as low temperatures and explosion-proof packaging). Sebaliknya, alcohol-free reagents eliminate these safety hazards through alternative formulations(such as polyethylene glycol and oil phases), reducing transportation costs and enhancing the stability of the reagent after opening.

Improving operational convenience and compatibility with automation, alcohol-containing reagents require manual intervention for addition in automated processes, whereas alcohol-free reagents can be preloaded directly into the kit, enabling fully enclosed automated operations. Misalnya, a certain blood nucleic acid extraction kit optimizes the lysis and rinse solution formulations to complete the magnetic bead-bound nucleic acid step without pausing the instrument, reducing the extraction time to within 20 menit.

Reducing residual interference, ethanol residues can inhibit downstream PCR and other enzymatic reactions, whereas alcohol-free reagents effectively remove impurities through washing liquid stratification techniques (such as the synergy between aqueous and oily phases), avoiding the negative impact of residues on detection sensitivity. Misalnya, a patented technology achieves efficient nucleic acid purification by washing with PEG in one step, increasing the recovery rate by four times compared to traditional methods.

Simplifying the formula and reducing costs, traditional alcohol-free solutions often require complex components (such as proteinase K). Sebaliknya, new reagents reduce the number of reagents and lower production costs by optimizing PEG concentration and sodium salt ratios. Misalnya, a magnetic bead kit only requires lysis buffer, wash buffer A/B, and elution buffer, eliminating the need for additional proteinase K, significantly reducing the complexity of the kit.

To enhance environmental friendliness, alcohol-free reagents avoid the use of toxic organic solvents, in line with the trend of green chemistry.

Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 yang merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami .