Produsen Profesional Manik Biomagnetik
Menggunakan Manik Magnetik Berbasis Silika untuk Mengekstraksi DNA/RNA dari Darah Utuh
1.Perkenalan
With the rapid development of biotechnology and the advancement of precision medicine, permintaan pasarnya terus meningkat. Shanghai Lingjun Bioteknologi Co., manik-manik magnetik Ltd (Nomor Produk. 160014) offer unique advantages in whole blood genomic DNA extraction kits.
In fields such as molecular diagnostics, genomics research, and viral detection, the efficient and high-purity extraction of nucleic acids (DNA/RNA) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. Karena itu, whole blood genomic DNA extraction has broad application prospects in multiple areas.
2. Silica-based magnetic beads
Itu 160014 manik-manik magnetik, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, penyuburan, and purification of genomic nucleic acids from fresh, beku, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. Itu 160014 magnetic beads specifically adsorb the nucleic acids, memisahkannya dari kotoran lain dalam sampel. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, nucleic acid detection analysis, deteksi mutasi gen, pengurutan, dan eksperimen lainnya.
3. Our product offers the following advantages
1、Untuk sampel yang sulit dilisiskan, itu 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.
2、The extraction process does not require the addition of Proteinase K, allowing for transportation and storage at room temperature.
3、The extracted genomic DNA exhibits high purity, hasil tinggi, dan pita elektroforesis utuh.
4、The extraction efficiency is high, with the entire process taking approximately 20 menit, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.
5、Kompatibilitas yang kuat, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.
4. Experimental Case
Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 manik-manik magnetik:
| Nomor sampel | Sample volume（μL） | A260/A280 | A260/A230 | Kesimpulan(ng/μL) | Elution volume（μL） | Nucleic acid yield（μg） |
| Mencicipi 1 | 200 | 1.950 | 1.493 | 59.10 | 80 | 4.728 |
| Mencicipi 2 | 1.964 | 1.523 | 60.65 | 4.852 | ||
| Mencicipi 3 | 1.928 | 1.469 | 74.30 | 5.944 | ||
| Mencicipi 4 | 1.967 | 1.481 | 62.75 | 5.020 | ||
| Mencicipi 5 | 1.982 | 1.797 | 81.30 | 6.504 | ||
| Mencicipi 6 | 2.006 | 1.890 | 78.75 | 6.300 | ||
| Mencicipi 7 | 1.932 | 1.524 | 66.10 | 5.288 | ||
| Mencicipi 8 | 1.945 | 1.540 | 72.90 | 5.832 | ||
| Mencicipi 9 | 1.956 | 1.728 | 99.90 | 7.992 | ||
| Mencicipi 10 | 1.991 | 1.832 | 88.80 | 7.104 | ||
| Mencicipi 11 | 1.943 | 1.485 | 78.10 | 6.248 | ||
| Mencicipi 12 | 1.975 | 1.473 | 38.15 | 3.052 | ||
| Mencicipi 13 | 1.917 | 1.459 | 105.80 | 8.464 | ||
| Mencicipi 14 | 1.988 | 1.657 | 58.05 | 4.644 | ||
| AVG | 1.963 | 1.578 | 70.37 | 5.630 |
Elektroforesis gel agarosa:
Analisis Hasil:
1、The extraction time is approximately 20 menit, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.
2、The extracted products show high purity: itu 260/280 ratios all reached above 1.9, dan itu 260/230 ratios all reached above 1.4.
3、The extracted products exhibit high yield: the yield from 200 μL samples was above 3 μg, meeting the requirements for downstream applications.
4、The electrophoresis bands are intact, with no degradation or smearing.
5. Aplikasi
High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, termasuk:
1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.
2、Pathogen Detection: Quantitative detection of viruses (misalnya, HBV, HCV, HIV) and bacterial load.
3、Forensik: STR analysis and DNA fingerprinting.
4、Urutan Generasi Berikutnya (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.
5、Biopreservation: Long-term storage of high-quality DNA.
Pemasok
Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 yang merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.
Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.
Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami .
