Profesjonell produsent av biomagnetiske perler
Bruk av silikabaserte magnetiske perler for å trekke ut DNA/RNA fra fullblod
1.Introduksjon
With the rapid development of biotechnology and the advancement of precision medicine, etterspørselen i markedet fortsetter å vokse. Shanghai Lingjun Biotechnology Co., Ltd.s magnetiske perler (Produktnr. 160014) offer unique advantages in whole blood genomic DNA extraction kits.
In fields such as molecular diagnostics, genomics research, and viral detection, the efficient and high-purity extraction of nucleic acids (DNA/RNA) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. Derfor, whole blood genomic DNA extraction has broad application prospects in multiple areas.
2. Silikabaserte magnetiske perler
De 160014 magnetiske perler, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, berikelse, og rensing av genomiske nukleinsyrer fra ferske, frossen, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. De 160014 magnetic beads specifically adsorb the nucleic acids, skille dem fra andre urenheter i prøven. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, nucleic acid detection analysis, påvisning av genmutasjoner, sekvensering, og andre eksperimenter.
3. Our product offers the following advantages
1、For difficult-to-lyse samples, de 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.
2、Ekstraksjonsprosessen krever ikke tilsetning av Proteinase K, allowing for transportation and storage at room temperature.
3、Det ekstraherte genomiske DNAet viser høy renhet, høyt utbytte, og intakte elektroforesebånd.
4、The extraction efficiency is high, with the entire process taking approximately 20 minutter, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.
5、Sterk kompatibilitet, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.
4. Experimental Case
Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 magnetiske perler:
| Eksempelnummer | Prøvevolum (μL） | A260/A280 | A260/A230 | Kons.(ng/μL) | Elueringsvolum (μL） | Nukleinsyreutbytte (μg） |
| Prøve 1 | 200 | 1.950 | 1.493 | 59.10 | 80 | 4.728 |
| Prøve 2 | 1.964 | 1.523 | 60.65 | 4.852 | ||
| Prøve 3 | 1.928 | 1.469 | 74.30 | 5.944 | ||
| Prøve 4 | 1.967 | 1.481 | 62.75 | 5.020 | ||
| Prøve 5 | 1.982 | 1.797 | 81.30 | 6.504 | ||
| Prøve 6 | 2.006 | 1.890 | 78.75 | 6.300 | ||
| Prøve 7 | 1.932 | 1.524 | 66.10 | 5.288 | ||
| Prøve 8 | 1.945 | 1.540 | 72.90 | 5.832 | ||
| Prøve 9 | 1.956 | 1.728 | 99.90 | 7.992 | ||
| Prøve 10 | 1.991 | 1.832 | 88.80 | 7.104 | ||
| Prøve 11 | 1.943 | 1.485 | 78.10 | 6.248 | ||
| Prøve 12 | 1.975 | 1.473 | 38.15 | 3.052 | ||
| Prøve 13 | 1.917 | 1.459 | 105.80 | 8.464 | ||
| Prøve 14 | 1.988 | 1.657 | 58.05 | 4.644 | ||
| AVG | 1.963 | 1.578 | 70.37 | 5.630 |
Agarosegelelektroforese:
Analysis of Results:
1、The extraction time is approximately 20 minutter, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.
2、The extracted products show high purity: de 260/280 ratios all reached above 1.9, og den 260/230 ratios all reached above 1.4.
3、The extracted products exhibit high yield: the yield from 200 μL samples was above 3 μg, meeting the requirements for downstream applications.
4、The electrophoresis bands are intact, with no degradation or smearing.
5. Søknad
High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, including:
1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.
2、Pathogen Detection: Quantitative detection of viruses (f.eks., HBV, HCV, HIV) and bacterial load.
3、Forensics: STR analysis and DNA fingerprinting.
4、Neste generasjons sekvensering (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.
5、Biopreservation: Long-term storage of high-quality DNA.
Leverandør
Shanghai Lingjun Biotechnology Co., Ltd.ble etablert i 2016 som er en profesjonell produsent av biomagnetiske materialer og nukleinsyreekstraksjonsreagenser.
Vi har rik erfaring innen utvinning og rensing av nukleinsyre, proteinrensing, celleseparasjon, kjemiluminescens, og andre tekniske felt.
Våre produkter er mye brukt på mange felt, som medisinsk testing, genetisk testing, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke bare produkter, men kan også påta oss OEM, ODM, og andre behov. Hvis du har et relatert behov, ta gjerne kontakt med oss .
