Professionel producent af biomagnetiske perler
Brug af silica-baserede magnetiske perler til at udtrække DNA/RNA fra fuldblod
1.Indledning
With the rapid development of biotechnology and the advancement of precision medicine, dets markedsefterspørgsel fortsætter med at vokse. Shanghai Lingjun Biotechnology Co., Ltd.s magnetiske perler (Produktnr. 160014) offer unique advantages in whole blood genomic DNA extraction kits.
In fields such as molecular diagnostics, genomisk forskning, and viral detection, the efficient and high-purity extraction of nucleic acids (DNA/RNA) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. Derfor, whole blood genomic DNA extraction has broad application prospects in multiple areas.
2. Silica-baserede magnetiske perler
De 160014 magnetiske perler, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, berigelse, and purification of genomic nucleic acids from fresh, frossen, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. De 160014 magnetic beads specifically adsorb the nucleic acids, adskille dem fra andre urenheder i prøven. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, nukleinsyredetektionsanalyse, genmutationspåvisning, sekventering, og andre eksperimenter.
3. Our product offers the following advantages
1、Til prøver, der er svære at lysere, de 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.
2、The extraction process does not require the addition of Proteinase K, allowing for transportation and storage at room temperature.
3、The extracted genomic DNA exhibits high purity, højt udbytte, og intakte elektroforesebånd.
4、The extraction efficiency is high, with the entire process taking approximately 20 minutter, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.
5、Stærk kompatibilitet, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.
4. Experimental Case
Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 magnetiske perler:
| Prøvenummer | Sample volume（μL） | A260/A280 | A260/A230 | Konc.(ng/μL) | Elution volume（μL） | Nucleic acid yield（μg） |
| Prøve 1 | 200 | 1.950 | 1.493 | 59.10 | 80 | 4.728 |
| Prøve 2 | 1.964 | 1.523 | 60.65 | 4.852 | ||
| Prøve 3 | 1.928 | 1.469 | 74.30 | 5.944 | ||
| Prøve 4 | 1.967 | 1.481 | 62.75 | 5.020 | ||
| Prøve 5 | 1.982 | 1.797 | 81.30 | 6.504 | ||
| Prøve 6 | 2.006 | 1.890 | 78.75 | 6.300 | ||
| Prøve 7 | 1.932 | 1.524 | 66.10 | 5.288 | ||
| Prøve 8 | 1.945 | 1.540 | 72.90 | 5.832 | ||
| Prøve 9 | 1.956 | 1.728 | 99.90 | 7.992 | ||
| Prøve 10 | 1.991 | 1.832 | 88.80 | 7.104 | ||
| Prøve 11 | 1.943 | 1.485 | 78.10 | 6.248 | ||
| Prøve 12 | 1.975 | 1.473 | 38.15 | 3.052 | ||
| Prøve 13 | 1.917 | 1.459 | 105.80 | 8.464 | ||
| Prøve 14 | 1.988 | 1.657 | 58.05 | 4.644 | ||
| AVG | 1.963 | 1.578 | 70.37 | 5.630 |
Agarosegelelektroforese:
Analyse af resultater:
1、The extraction time is approximately 20 minutter, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.
2、The extracted products show high purity: de 260/280 ratios all reached above 1.9, og den 260/230 ratios all reached above 1.4.
3、The extracted products exhibit high yield: the yield from 200 μL samples was above 3 μg, meeting the requirements for downstream applications.
4、The electrophoresis bands are intact, with no degradation or smearing.
5. Anvendelse
High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, inklusive:
1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.
2、Påvisning af patogener: Quantitative detection of viruses (f.eks., HBV, HCV, HIV) and bacterial load.
3、Forensics: STR analysis and DNA fingerprinting.
4、Næste generations sekvensering (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.
5、Biopreservation: Long-term storage of high-quality DNA.
Leverandør
Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.
Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens, og andre tekniske områder.
Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har et relateret behov, er du velkommen til at kontakte os .
