Uso de perlas magnéticas a base de sílice para extraer ADN/ARN de sangre total

1.Introducción

With the rapid development of biotechnology and the advancement of precision medicine, su demanda en el mercado sigue creciendo. Shanghai Lingjun Biotecnología Co., Cuentas magnéticas de Ltd. (Número de producto. 160014) offer unique advantages in whole blood genomic DNA extraction kits.

In fields such as molecular diagnostics, investigación genómica, and viral detection, the efficient and high-purity extraction of nucleic acids (ADN/ARN) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. Por lo tanto, whole blood genomic DNA extraction has broad application prospects in multiple areas.

2. Perlas magnéticas a base de sílice

El 160014 cuentas magnéticas, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, enriquecimiento, and purification of genomic nucleic acids from fresh, congelado, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. El 160014 magnetic beads specifically adsorb the nucleic acids, Separándolos de otras impurezas en la muestra.. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, análisis de detección de ácidos nucleicos, detección de mutaciones genéticas, secuenciación, y otros experimentos.

3. Our product offers the following advantages

1、Para muestras difíciles de lisar, el 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.

2、The extraction process does not require the addition of Proteinase K, allowing for transportation and storage at room temperature.

3、The extracted genomic DNA exhibits high purity, alto rendimiento, y bandas de electroforesis intactas.

4、The extraction efficiency is high, with the entire process taking approximately 20 minutos, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.

5、Strong compatibility, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.

4. Caso experimental

 Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 cuentas magnéticas:

Número de muestra	Sample volume（µL）	A260/A280	A260/A230	Conc.(ng/μL)	Elution volume（µL）	Nucleic acid yield（µg）
Muestra 1	200	1.950	1.493	59.10	80	4.728
Muestra 2		1.964	1.523	60.65		4.852
Muestra 3		1.928	1.469	74.30		5.944
Muestra 4		1.967	1.481	62.75		5.020
Muestra 5		1.982	1.797	81.30		6.504
Muestra 6		2.006	1.890	78.75		6.300
Muestra 7		1.932	1.524	66.10		5.288
Muestra 8		1.945	1.540	72.90		5.832
Muestra 9		1.956	1.728	99.90		7.992
Muestra 10		1.991	1.832	88.80		7.104
Muestra 11		1.943	1.485	78.10		6.248
Muestra 12		1.975	1.473	38.15		3.052
Muestra 13		1.917	1.459	105.80		8.464
Muestra 14		1.988	1.657	58.05		4.644
AVG		1.963	1.578	70.37		5.630

Electroforesis en gel de agarosa:

Análisis de resultados:

1、The extraction time is approximately 20 minutos, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.

2、The extracted products show high purity: el 260/280 ratios all reached above 1.9, y el 260/230 ratios all reached above 1.4.

3、The extracted products exhibit high yield: the yield from 200 μL samples was above 3 µg, meeting the requirements for downstream applications.

4、The electrophoresis bands are intact, with no degradation or smearing.

5. Solicitud

High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, incluido:

1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.

2、Detección de patógenos: Quantitative detection of viruses (p.ej., VHB, HCV, HIV) and bacterial load.

3、Forensics: STR analysis and DNA fingerprinting.

4、Secuenciación de próxima generación (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.

5、Biopreservation: Long-term storage of high-quality DNA.

Proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 que es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes una necesidad relacionada, no dude en contactarnos .