실리카 기반 자기 비드를 사용하여 전혈에서 DNA/RNA 추출

1.소개

With the rapid development of biotechnology and the advancement of precision medicine, 시장 수요는 계속해서 증가하고 있다. 상하이 링쥔 생명공학 유한회사, (주)자성비즈 (제품 번호. 160014) offer unique advantages in whole blood genomic DNA extraction kits.

In fields such as molecular diagnostics, genomics research, and viral detection, the efficient and high-purity extraction of nucleic acids (DNA/RNA) from complex samples—especially whole blood—is a critical first step for the success of downstream experiments. 그러므로, whole blood genomic DNA extraction has broad application prospects in multiple areas.

2. 실리카 기반 자기 비드

그만큼 160014 자기 구슬, used in conjunction with Lingjun’s Whole Blood Genomic DNA Extraction Kit, are designed for the extraction, 농축, 신선한 시료로부터 게놈 핵산을 정제, 언, or anticoagulant-treated blood samples. Under the action of guanidine salts, sample cells are lysed to release nucleic acids. 그만큼 160014 magnetic beads specifically adsorb the nucleic acids, 시료의 다른 불순물과 분리. After washing with a buffer to remove proteins and residual guanidine salts, the purified nucleic acids are eluted and collected via magnetic adsorption. The resulting product can be used in PCR amplification, 핵산 검출 분석, 유전자 돌연변이 검출, 시퀀싱, 그리고 다른 실험들.

3. Our product offers the following advantages

1、For difficult-to-lyse samples, 그만큼 160014 magnetic beads combined with the extraction kit enable simultaneous lysis and binding without the need to pause and add other reagents midway.

2、추출 공정에는 Proteinase K를 첨가할 필요가 없습니다., allowing for transportation and storage at room temperature.

3、추출된 genomic DNA는 높은 순도를 나타냅니다., 높은 수율, 손상되지 않은 전기영동 밴드.

4、The extraction efficiency is high, with the entire process taking approximately 20 분, making it suitable for extracting nucleic acids from 200–400 μL of whole blood samples.

5、강력한 호환성, allowing it to be used with automated nucleic acid extraction equipment from various manufacturers.

4. Experimental Case

 Experimental results of extracting genomic DNA from fresh blood samples of different healthy individuals using 160014 자기 구슬:

샘플 번호	샘플량(μL)	A260/A280	A260/A230	농도(ng/μL)	용출량（μL)	핵산 수율（μg)
견본 1	200	1.950	1.493	59.10	80	4.728
견본 2		1.964	1.523	60.65		4.852
견본 3		1.928	1.469	74.30		5.944
견본 4		1.967	1.481	62.75		5.020
견본 5		1.982	1.797	81.30		6.504
견본 6		2.006	1.890	78.75		6.300
견본 7		1.932	1.524	66.10		5.288
견본 8		1.945	1.540	72.90		5.832
견본 9		1.956	1.728	99.90		7.992
견본 10		1.991	1.832	88.80		7.104
견본 11		1.943	1.485	78.10		6.248
견본 12		1.975	1.473	38.15		3.052
견본 13		1.917	1.459	105.80		8.464
견본 14		1.988	1.657	58.05		4.644
AVG		1.963	1.578	70.37		5.630

아가로스 겔 전기영동:

결과 분석:

1、The extraction time is approximately 20 분, and genomic DNA was successfully extracted from all 14 human whole blood samples with high stability.

2、The extracted products show high purity: 그만큼 260/280 ratios all reached above 1.9, 그리고 260/230 ratios all reached above 1.4.

3、The extracted products exhibit high yield: the yield from 200 μL 샘플이 위에 있었습니다. 3 μg, meeting the requirements for downstream applications.

4、The electrophoresis bands are intact, 분해나 번짐 없이.

5. 애플리케이션

High-quality nucleic acids extracted from whole blood can be used in various advanced molecular biology applications, 포함:

1、Genetic Testing: Such as genetic disease screening and pharmacogenomics.

2、병원체 검출: Quantitative detection of viruses (예를 들어, HBV, HCV, HIV) and bacterial load.

3、법의학: STR analysis and DNA fingerprinting.

4、차세대 시퀀싱 (NGS): Whole genome sequencing (WGS) and targeted sequencing, which require inhibitor-free and intact input DNA.

5、Biopreservation: Long-term storage of high-quality DNA.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .