겨울철 수동 마그네틱 비드 추출: 저온 취급 시 주요 주의사항

머리말

When manually using magnetic bead – based nucleic acid extraction reagents in cold winter, special attention should be paid to the impact of temperature on reagent activity, magnetic bead adsorption efficiency, and experimental operations. The specific precautions are as follows:

1. Reagent Rewarming in Advance

Reagents such as magnetic bead suspensions, lysis buffers, and washing buffers are prone to component precipitation or magnetic bead aggregation at low temperatures. Take them out of the refrigerator 15 – 30 minutes in advance, let them rewarm at room temperature, and gently invert and mix them. Avoid violent shaking to prevent magnetic beads from breaking.

2. Maintain a Stable Operating Environment Temperature

Try to operate in a laboratory with a room temperature of 18 – 25℃. Avoid areas directly blown by cold air, 공기와 같은 – conditioner outlets and doors and windows. If the ambient temperature is too low, use a constant – temperature metal bath or water bath to maintain the temperature of samples and reagents, preventing a decrease in the efficiency of the lysis buffer and insufficient binding between DNA and magnetic beads.

3. Control Time in Magnetic Bead Adsorption and Separation Steps

Low temperatures will slow down the binding speed of magnetic beads and nucleic acids. Appropriately extend the incubation time of magnetic beads (usually increase by 5 – 10 분). 동시에, when separating magnetic beads on the magnetic stand, the sedimentation speed of magnetic beads slows down at low temperatures. Prolong the standing time to ensure that the magnetic beads are completely adsorbed on the tube wall before discarding the waste liquid to avoid the loss of magnetic beads.

4. Optimize the Temperature of the Eluent

The temperature of the eluent directly affects the nucleic acid elution efficiency. In winter, pre – warm the eluent to 50 – 65℃ before use. Briefly vortex and mix during elution and let it stand for 2 – 3 minutes to improve the nucleic acid recovery rate. The eluted nucleic acid sample should be stored at a low temperature as soon as possible to prevent degradation.

5. Operational Details and Consumable Preparation

Pre – warm the pipette in advance to avoid inaccurate liquid aspiration by the pipette at low temperatures. Use enzyme – free centrifuge tubes and pipette tips to prevent the loss of nucleic acids adsorbed on the surface of consumables in a low – temperature environment. Operate gently to reduce reagent volatilization and sample contamination.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .